UHPLC and HPLC Products & Reviews

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High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Scientific™ Acclaim™ 120 C18 Reversed-Phase LC Column

Thermo Fisher Scientific

High Performance Reversed-Phase Columns for the Separation of Small Molecules. The Acclaim® 120 columns are designed for high resolution reversed-phase separations. The very high surface coverage and very low metal content together result in columns with excellent efficiencies. These columns provide exceptional performance for a variety of applications in the pharmaceutical, chemical, environmental, and food separations areas.…

4.5/5.0
|4 Reviews
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Thermo Scientific™ Acclaim Carbamate LC Column

Thermo Fisher Scientific

The Acclaim Carbamate column is designed for baseline separation of carbamates (N-methylcarbamate and N-methylcarbamoyloxime pesticides) specified in US EPA Method 531.2. Carbamate pesticides are widely used throughout the world. Drinking water and raw surface water is monitored for the presence of carbamate pesticides and related compounds using an established EPA Method 531.2 that uses HPLC with postcolumn derivatization.…

5.0/5.0
|1 Review
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Thermo Scientific™ DNAPac™ PA200 Nucleic Acid Column

Thermo Fisher Scientific

Ultrahigh-Resolution Separations of OligonucleotidesThe Thermo Scientific™ DNAPac™ PA200 and PA200 RS are strong anion-exchange columns developed to provide unsurpassed high-resolution analysis and purification of synthetic oligonucleotides. Both supports are designed to resolve full length from n–1, n+1, and other failure sequences not possible with other columns. Retention times and selectivity can be controlled by choice of…

5.0/5.0
|1 Review
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Thermo Scientific™ MAbPac™ SEC-1 Monoclonal Antibody Analysis Column

Thermo Fisher Scientific

MAbPac™ SEC– 1 for Monoclonal Antibody (MAb) Analysis MAbPac™ SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers, aggregates, and fragments, under non-denaturing conditions, and in both high- and low-salt mobile phases and volatile eluents.SEC is frequently used because of its simple operating principle and features not found in other sep…

5.0/5.0
|1 Review
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Thermo Scientific™ Vanquish™ Flex Quaternary UHPLC System

Thermo Fisher Scientific

The Thermo Scientific™ Vanquish™ Flex Binary UHPLC system is biocompatible with a state-of-the-art binary high-pressure pump and state-of-the-art binary solvent blending. This ultra high performance liquid chromatography system shares all Vanquish values, such as a design focused on uptime, robustness and reliability. Multiple detection options as well as Thermo Scientific™ Viper™ fingertight connections throughout the system…

4.7/5.0
|14 Reviews
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Thermo Scientific™ MAbPac™ RP LC Columns

Thermo Fisher Scientific

Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized to for high resolut…

5.0/5.0
|1 Review
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Thermo Scientific™ Vanquish™ Variable Wavelength Detector F

Thermo Fisher Scientific

Achieve excellent linearity and noise performance to support a wide operating range and trace detection using the Thermo Scientific™ Vanquish™ Variable Wavelength Detector F. This detector is integrated in the Vanquish UHPLC system when you need more versatility, and robust and high-performance UV-Vis absorption detection to measure your sample. With up to 250 Hz, the detector keeps pace with any current and future UHPLC detec…

4.7/5.0
|1 Review
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Thermo Scientific™ Vanquish™ Fluorescence Detector F

Thermo Fisher Scientific

Get maximum fluorescence detection effectiveness from the sophisticated design of multiple measurement features in the Thermo Scientific™ Vanquish™ Fluorescence Detector. Monitor up to four excitation/emission wavelength pairs simultaneously in multichannel operation mode with ultrafast wavelength switching, or scan your chromatogram for the best emission/excitation wavelengths with fluorescence fields. The optical design prov…

4.9/5.0
|2 Reviews
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Thermo Scientific™ Vanquish™ Horizon UHPLC System

Thermo Fisher Scientific

The Thermo Scientific™ Vanquish™ Horizon UHPLC System is built from the ground up to deliver better results with superior separations and easier interaction with no trade-offs in performance, robustness, or ease-of-use. This fully integrated and biocompatible system features high sample capacity for high-throughput workflows, industry-leading pumping performance, amazingly high signal-to-noise (S/N) and linearity, two-mode the…

5.0/5.0
|1 Review
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Thermo Scientific™ Vanquish™ Duo UHPLC System for Dual LC

Thermo Fisher Scientific

Improve your productivity and sample knowledge for a faster return on investment with the Thermo Scientific™ Vanquish™ Duo UHPLC systems. Vanquish Duo UHPLC systems use a separate second flow path in one integrated system. With the Thermo Scientific™ Vanquish™ Duo UHPLC system for Dual LC, double your throughput or deepen your sample knowledge. Run the same method on both flow paths or two different methods simultaneously on o…

4.7/5.0
|14 Reviews
  • Best New Separations Product of the Year
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Thermo Scientific™ UltiMate™ 3000 Biocompatible Rapid Separation (BioRS) Dual System

Thermo Fisher Scientific

Speed up Bio-UHPLC workflows without redeveloping existing methods. The Thermo Scientific™ UltiMate™ 3000 BioRS Dual System offers outstanding LC and advanced bio-UHPLC capabilities. Two independently operating gradient pumps and smart column switching save time. Offline fractionation combines noncompatible chemistries in one workflow. With our unique fractionation autosampler, the system can completely automate multiple UHPLC…

5.0/5.0
|1 Review
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Thermo Scientific™ UltiMate™ 3000 Manual Basic LC System

Thermo Fisher Scientific

Enjoy cost effective access to UHPLC separations in a simple, expandable system where modularity makes anything possible. The Thermo Scientific™ UltiMate™ 3000 Basic Manual system offers unique LC and entry level UHPLC capabilities without compromising on quality and robustness. The basic manual system is perfect for teaching or low sample throughput, routine applications. As an LC/UHPLC “starter kit”, this modular system can…

5.0/5.0
|1 Review
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Thermo Scientific™ UltiMate™ LPG-3400SD Standard Quaternary Pump

Thermo Fisher Scientific

For flexibility across a broad range of analytical LC applications with the highest degree of choice in solvent proportioning and mixing, the Thermo Scientific™ UltiMate™ LPG-3400SD Standard Quaternary Pump is the pump of choice. Up to four solvents may be used for convenient method development, system flushing or solvent preparation. With support for pressures up to 62 MPa (9,000 psi/620 bar) at flow rates of up to 10mL/min.,…

5.0/5.0
|2 Reviews
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DAWN™

Waters | Wyatt Technology

The DAWN is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering the highest sensitivity and widest range of molecular weight, size, concentration and optional features.

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miniDAWN™

Waters | Wyatt Technology

The miniDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering superb sensitivity over a wide range of molecular weight, size and concentration.

4.7/5.0
|42 Reviews
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