PCR and Thermal Cycling Products & Reviews

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Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Air-Dryable Probe Mix

PCR Biosystems

Air-Dryable Probe Mix is a versatile, air-dryable 4x qPCR reagent mix for ultra-sensitive detection of DNA sequences. The mix is glycerol-free and contains hot start Taq polymerase, dNTPs, Mg and a blend of excipients to ensure reliable air-drying, without loss of activity.

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Clara® Inhibitor-Tolerant Probe Mix

PCR Biosystems

Unlock the power of precise qPCR analysis with Clara® Inhibitor-Tolerant Probe Mix. This broad-spectrum, inhibitor-tolerant qPCR mix is designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for your research.

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Clara® Probe 1-Step Purple Mix

PCR Biosystems

Clara® Probe 1-Step Purple Mix offers clear and consistent results every time, with an easy-to-see, inert, purple dye for sample visualisation. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

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Air-Dryable Inhibitor-Tolerant Probe 1-Step Mix

PCR Biosystems

Air-Dryable Inhibitor-Tolerant Probe 1-Step Mix is a glycerol-free 4x RT-qPCR mix that combines our novel inhibitor-tolerant chemistry alongside our cutting-edge air-dryable technology. Now you can benefit from dried, room temperature stable qPCR assays capable of highly-sensitive RNA & DNA detection on the most challenging, inhibitor-rich sample types.

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Air-Dryable Inhibitor-Tolerant Probe Mix

PCR Biosystems

Air-Dryable Inhibitor-Tolerant Probe Mix is a glycerol-free 4x qPCR mix that combines our novel inhibitor-tolerant chemistry alongside our cutting-edge air-dryable technology. Now you can benefit from dried, room-temperature stable qPCR assays capable of highly-sensitive DNA detection on the most challenging, inhibitor-rich sample types.

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Clara® Inhibitor-Tolerant Probe 1-Step Mix

PCR Biosystems

Unlock the power of precise RT-qPCR analysis with Clara® Inhibitor-Tolerant Probe 1-Step Mix. This broad-spectrum, inhibitor-tolerant RT-qPCR mix is designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for your research.

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PCRBIO Taq Mix

PCR Biosystems

PCRBIO Taq Mix is an affordable, versatile and robust 2x mix for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step polymerase purification strategy together with an optimised buffer system enable PCRBIO Taq Mix to amplify with the highest speed, yield and specificity.

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PCRBIO HS Taq Mix

PCR Biosystems

PCRBIO HS Taq Mix is an advanced antibody-mediated hot start DNA polymerase mix designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

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PCRBIO HS Taq DNA Polymerase

PCR Biosystems

PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start DNA polymerase designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

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UltraScript® 2.0 Reverse Transcriptase

PCR Biosystems

UltraScript® 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase designed for cDNA synthesis of the most challenging RNA sample types. This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55 °C, giving improved specificity, higher cDNA yields and more full length cDNA product.

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qPCRBIO SyGreen® Blue Mix

PCR Biosystems

qPCRBIO SyGreen® Blue Mix uses a non-reactive blue dye for easy sample visualisation during reaction setup, allowing fast, highly sensitive and reproducible real-time PCR with more precision and accuracy than ever. This easy-to-see mix is particularly useful in reducing errors when loading small volume reactions or using white plates.

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VeriFi® Mix

PCR Biosystems

VeriFi® Mix is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. 

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VeriFi® Hot Start Mix

PCR Biosystems

VeriFi® Hot Start Mix is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

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VeriFi® Hot Start Mix Red

PCR Biosystems

VeriFi® Hot Start Mix Red is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. The mix includes a convenient red dye for direct gel loading.

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VeriFi® Hot Start Polymerase

PCR Biosystems

VeriFi® Hot Start Polymerase is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

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qPCRBIO Probe Blue Mix

PCR Biosystems

qPCRBIO Probe Blue Mix is a universal probe kit that includes a non-reactive blue dye for easy sample visualisation and enhanced pipetting accuracy. Whether your application is for a singleplex or multiplex expression study or for genotyping and allelic discrimination analyses, qPCRBIO Probe Blue Mix is the robust choice for all your probe-based real-time PCR needs.

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PCRBIO HiFi Polymerase

PCR Biosystems

PCRBIO HiFi Polymerase is our original high-fidelity enzyme, derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.

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PCRBIO Ultra Polymerase

PCR Biosystems

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

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PCRBIO Ultra Mix

PCR Biosystems

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

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