PCR and Thermal Cycling Products & Reviews

Our new Service Program ensures you have a fully functional Mx3000P or Mx3005P QPCR System within 2 business days of reporting a problem. This maximizes the amount of time your QPCR system is up and running, ensuring an uninterrupted workflow and quicker response time than any other service program currently available in the industry.  Factory Service Center repair with loaner instrument for duration of repair  Guaranteed l…

The Agilent High-Specificity miRNA QRT-PCR Detection Kit and miRNA QRT-PCR Master Mix Detection Kit provide qualified reagents to polyadenylation of microRNAs (miRNAs) followed by synthesis of first-strand cDNA from these tailed miRNAs. A novel PCR enzyme formulation and qPCR detection reagents give utmost specificity.

The universal reverse primer is designed for use with the Human U6 forward primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work. The Kit delivers sensitive and reproducible detection of miRNA with high specificity.

Our miRNA qRT-PCR detection system enables accurate and real-time PCR quantification for miRNA. The kit is a high-specificity reagent system that delivers sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation reaction and then reverse transcribes the polyadenylated RNA into QPCR-ready cDNA.

Agilent miRNA qPCR Detection Reagents are high-specificity reagents for the polyadenylation of microRNAs (miRNAs) from total RNA. The miRNA qPCR Detection Reagents also enable the synthesis of first-strand cDNA from the tailed miRNAs.

Our SideStep II QRT-PCR products deliver efficient cell lysis and sensitive quantitative gene expression analysis without RNA purification. Utilizing Agilent’s Brilliant QRT-PCR technology, multiple samples can be screened quickly and easily. The one-step process results in RNA stabilized for up to 6 months.

Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries.  Makes blunt double-stranded cDNA  Can be used for in vitro transcription  Perfect for nondirectional cDNA libraries

QuikHyb hybridization solution was developed for use in Southern, Northern, slot-blot and plaque-lift formats using randomly labeled radioactive and nonradioactive nucleic acid probes.  Reduces hybridization time from customary 12-24 hours to 1-2 hours  Efficient and sensitive radioactive and nonradioactive detection of nucleic acids  Doubles signal intensity

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Agilent miRNA Kit may also be used as an optimization or standardization reagent for human miRNA analysis. Available in industrial lots for high throughout applications.

Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.

The Agilent SARS-CoV-2 qRT-PCR Dx kit is a real-time reverse transcriptase PCR (qRT-PCR) in vitro diagnostic test for qualitative detection of SARS-CoV-2 RNA.

Expand your in-house food safety PCR testing capabilities with a high-performance benchtop platform: Carry out rapid food protection testing with the Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System. The affordable and easy-to-use system is ideal for users who need superior performance, maximum dye versatility, and security options in a real time PCR system.

Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test: The Thermo Scientific™ SureTect™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Assay enables you to rapidly identify and distinguish key Salmonella strains of concern in a single test.The workflows for testing poultry and pork meats, as well as production environment samples, ha…

Enhance your laboratory productivity and performance with the Thermo Scientific™ SureTect™ Automation Platform, utilizing the CyBio™ FeliX Instrument: Take your food safety testing to the next level with automated lysis and PCR preparation. The simple workflow and rapid setup allows you to reduce hands-on time while increasing the accuracy of your testing, enabling your technicians to focus on other tasks.

Threefold power for maximum flexibility in PCR. The thermal cycler Biometra TRIO provides three independent sample blocks which can run three different PCR reactions in parallel. The instrument is available with different sample blocks: 3x48 well for 0.2 ml tubes, 3x30 well for 0.5 ml tubes or 3x combi block for 48 well 0.2 ml tubes or 18x0.5 ml tubes each. Thus, this thermocycler offers high throughput in parallel operatio…

Bacteria Validated Rapid Detection Kit