Protein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-M, EDTA-free to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein e…
Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-M to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteas…
Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-Y, EDTA-free for the rapid cell lysis of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room tempera…
The Steptavidin is produced by fermentation of S. avidinii in a minimal medium. The protein has a structure with four identical subunits, each containing a binding site for biotin. It is stable over a wide range of pH and decomposes only in the presence of SDS at temperatures above 60 °C. Steptavidin conjugated with an enzyme, i.e. Horseradish Peroxidase or Alkaline Phosphatase or a fluorochrome may be used to visualize biot…
The Empore™ High Performance Extraction Disk Plate differs in design from plates offered by other manufacturers and requires a specific vacuum manifold. Manifolds supplied by different plate manufacturers are specific to the design of their plates and cannot generally be used with plates from other manufacturers. In some cases, the use of spacers to raise/lower the height of a collection device inside the manifold may overco…
Replacement gaskets are available with the vacuum manifold assembly. The gasket can become worn over time and the seal between the plate and the manifold will be affected. This may result in poor vacuum pressure and negatively effect flow rates. The gasket should be checked regularly and replaced when it becomes worn.
Agilent Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases.
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. The AbSelect™ Purification Kit quickly removes these contaminants of antibody labelling. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5m…
The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the highly selective and easily controllable interaction between the Strep-tag II peptide and the specially engineered streptavidin called Strep-Tactin. The binding affinity of Strep-tag II to Strep-Tactin is nearly 100 times higher than to Stre…
The recently launched Strep-Tactin® Superflow® high capacity resin offers improved binding capacity but identical high protein purity compared to standard Strep-Tactin® Superflow®. Thus, it is the perfect solution for Strep-tag® protein purification. Both Strep-Tactin® Superflow® resins • combine outstanding flow characteristics with superior mechanical stability • are suitable for increased flow rates and useful for purificat…
