cOmplete Lysis-Y, EDTA-free

Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC.

Application

Use Complete Lysis-Y, EDTA-free for the rapid cell lysis of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room temperature and the simultaneous inhibition of protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteases, including serine proteases and cysteine proteases, but not metalloproteases.

The EDTA-free version of Complete Protease Inhibitor Cocktail Tablets in the kit is used to inhibit proteolytic activity in cases in which EDTA may interfere with protein stability or subsequent assays or purification methods (e.g., Immobilized Metal Chelate Affinity Chromatography [IMAC]).

Benefits

• Save time with ready-to-use reagents for easy, fast, and gentle lysis of yeast cells in only 20 minutes.
• Obtain twice the yield of extracted proteins compared to glass bead methods.
• Ensure protein integrity by using reagents that do not denature or interact with proteins.
• Eliminate the need for glass beads.
• Achieve non-toxic, effective protection of proteins against a multitude of proteases (serine proteases and cysteine proteases, but not metalloproteases).
• Employ reagents that are suitable for Immobilized Metal Chelate Affinity Chromatography (IMAC) and downstream assays.

Product Description

Due to the complex and stable cell wall, yeast protein extraction is difficult and time consuming. The Complete Lysis-Y yeast-cell protein extraction reagent contains a mild ready-to-use solution that efficiently lyses the following organisms: Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Bacillus subtilis, eliminating the need for glass beads. Rapid cell lysis occurs in just 20 minutes at room temperature. The protein yields obtained with this kit are two times higher compared to those obtained by using glass beads.

In combination with Complete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets, included in the kit, the Lysis-Y Reagent provides a very convenient and efficient way to extract proteins and simultaneously inhibit protease activity during the initial extraction steps. Complete Tablets eliminate the time-consuming search for the right protease inhibitor. The ready-to-use water-soluble, non-toxic tablets work optimally with the kit’s Lysis-Y Reagent. The extracted proteins can be further purified using Immobilized Metal Chelate Affinity Chromatography (IMAC) or analyzed in downstream applications.

Contents

200 ml Lysis-Y Reagent for protein extraction from yeast cells
20 Complete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets supplied in EASYpacks (each tablet is sufficient for 10 ml of extract)