High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Spark Holland OPTIMAS 84 Position Autosampler. OPTIMAS features our proven PASA injection concept, combining robust injection with high precision and accuracy. With its three injection modes including zero sample loss injection, OPTIMAS rivals top-class autosampler performance, while challenging middle-class autosampler prices! Features: Reliable PASA injection technology Superior price/ performance ratio Sample c…
The SEDEX Model 75 provides the option of 4 new nebulizers and a redesigned evaporation tube. These changes allow you to use a lower temperature to evaporate the mobile phase, this provides a more uniform droplet size, maximizing the sensitivity and repeatability. Enhanced ease of operation The SEDEX Model 75 is designed to be very easy to use. The operator needs to set only three controls, the gain, the zero and the evaporati…
Use Thermo Scientific™ GlycanPac™ AXH-1 LC Columns for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules.This high-performance, silica-based high-performance liquid chromatography (HPLC) column provides simultaneous separation of glycans by charge, size and polarity. Achieve high-resolution and high-throughput analysis with unique selectivity for biologi…
Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical da…
For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. Featuring a lower hydrophobicity than C18 or C8 columns, Hypersil GOLD C4 columns provide outstanding sensitivity and high efficiency.
4.6mm, 4.0mm, 2.0mm, and 1.0mm ID HPLC columns are available in lengths of 50mm, 100mm, 150mm, and 250mm. SGE has biocompatible GLT columns available for applications requiring truly inert HPLC columns. SGE HPLC columns offer brilliant results for reverse phase, and normal phase HPLC applications.
The LC-4000 Series includes three PDA detectors.The MD - 4 017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhor se for UV- visible detection in the range 200 to 600nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution. The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detec tor. Making use of a 1024 eleme…
The FP-4020 fluorescence detector has been designed for the pursuit of extreme sensitivity.Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperature- regulated cell body has led to the highest sensitivity with stable detection. The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a gi…
The OR-4090 Chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption.The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV/Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of…
Visco Mixers are static mixers for HPLC and other high pressure applications. The Lee Visco-Jet Micro-Mixer uses aerospace technology to provide the ultimate in static mixing efficiency. A series of 36 critically controlled spin chambers subject the incoming liquids to a vigorously repeated mixing process. No electrical or mechanical input is required – the mixing energy is drawn from the liquids themselves. Two sizes ar…
Get more of what you want, and less of what you don't, with Roc™ LC columns from Restek.
Pirkle-brush Concept Chiral HPLC ColumnsChirex® HPLC columns utilizes Pirkle-brush concept for direct and indirect resolution of enantiomeric amines, alcohols, carboxylic acids, hydroxy acids, amino acids, ketones, lactones, ethers, esters, and other biologically active compounds. Chirex HPLC columns are available in eleven different stationary phases and offer:High enantioselectivityEasy scale-up from analytical to preparativ…
Guaranteed Replacement to Inertsil HPLC ColumnsProdigy™ columns have been developed as alternatives to the Inertsil® 3μ ODS-3, 5μ C8, C8-3, Phenyl-3, ODS-2, ODS-3 and ODS-3V packings. These phases represent a major advance in the analysis of basic, acidic and amphoteric compounds.
Purification and Analysis of Synthetic OligonucleotidesThe Clarity BioSolutions LC portfolio includes novel RP-LC and IEX-LC columns for purifying and characterizing synthetic DNA / RNA used in biological research, therapeutic development, and biochemical manufacturing.Clarity Oligo-MS™: LC/MS analysisClarity Oligo-RP™: RP-LC purification and analysisClarity Oligo-WAX™: IEX-LC purification
