High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Bonus-RP columns provide unique selectivity and applicability for mid pHs with an embedded amide linkage in the C14-alkyl chain. At pH 7 Bonus-RP provides excellent peak shape for even the most challenging basic compounds.
The 1290 Infinity II Refractive Index Detector (RID) is a UHPLC RID detector that provides excellent sensitivity, resolution, and sample throughput for UHPLC DRI experiments. The 1290 Infinity II RID is a equipped with an ultralow dispersion microflow cell and a data rate of 148 Hz, which significantly reduces run times for higher sample throughput and improved resolution and sensitivity.
The 1260 Infinity II Bio-Inert LC is a dedicated solution for analysis of large biomolecules in biological samples, including monoclonal antibody (mAb) and protein analysis. Metal-free components in the sample flow path and the absence of iron and steel in solvent delivery lines ensure the integrity of your biomolecules, minimize unwanted surface interactions, and increase column lifetime.
The 1260 Infinity II Degasser is suitable for analytical applications with flow rates up to 10 mL/min and up to four solvents. It is highly recommended for GPC/SEC applications using refractive index detection. It reduces baseline noise and quenching effects due to high degassing capacity.
The 1290 Infinity Flexible Cube is an auxiliary module that allows flexible online SPE applications by supporting up to two Agilent Quick Change Valve drives for online SPE valves and a built-in pump to facilitate enhanced flushing capabilities and to reduce carryover.
LC Manual Syringes with PTFE-Tipped Plungers
ZirChrom Separations Inc. (Anoka, MN) provides a full line of zirconia-based HPLC columns for chromatographic method development. ZirChrom HPLC columns offer unique selectivity, exacting reproducibility, high efficiency and ultra-stability. ZirChrom columns have proven utility over a wide range of pharmaceutical compounds. The columns may be used at both ambient and elevated temperatures (which helps enhance LC/MS sensitivi…
A Sensitive solution for HPLC, fast-LC and CE systems High Sensitivity: 10 times to 100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection. Versatility: 14 interchangelable different lasers availables for any application Modularity : Adaptable to CE and HPLC systems including Fast-LC, Capillary LC and nanoLC.Selectivity: Low noise and interference molecules will be detected.…
The ZORBAX Eclipse AAA high efficiency HPLC column rapidly separates amino acids following an updated and improved protocol. Total analysis from injection to injection can be achieved in as little as 14 min (10 min analysis time) on the short, 7.5 cm column and 24 min (16 min analysis time) on the 15 cm column length.Exceptional sensitivity (5 - 50 pmol with DAD, FLD) and reliability are achieved using both OPA and FMOC deriv…
Exceptional peak symmetry and resolution Based on next-generation ultra pure silica Outstanding pH stability Ideal first choice for new method developmentProduct detail:Hypersil GOLD columns are a new LC and LC/MS column offering unsurpassed peak shape. Based on highly pure silica, Hypersil GOLD columns provide the ultimate in symmetrical peaks, even when analyzing compounds that give notoriously poor peak shape on traditional…
