High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Kinetex PAH is the first polymerically bonded C18 core-shell column developed specifically for the analysis of PAHs from a wide variety of matrices. It can be used on both HPLC and UHPLC instrumentation and will easily shorten method run times, while improving overall resolution and sensitivity. To ensure a high standard of quality for the analysis of PAHs, Kinetex PAH is QC tested for the EPA 610 set of PAHs from water.
Despite being a drug of abuse in many countries, many societies are currently experiencing an increasingly rapid acceptance of cannabis (marijuana) products for medicinal and recreational usage. Additionally, low cannabis content and high hemp fibre products are grown in numerous countries. In addition to cannabinoid analysis of clinical samples, there is a need to determine the provenance, identification, purity and quant…
Do not waste time and labor with manual sample preparation. The AZURA GPC Cleanup system automates work-intensive and time-consuming cleanup tasks based on gel permeation chromatography (GPC). Further, reproducibility and quality of the cleanup are improved. After sample loading, the dedicated system fully automatically processes up to 15 samples while requiring minimal bench space. Based on the versatile AZURA device platform…
With an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradient solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
Flangeless Fittings eliminate the need to flange tubing. This removable and reusable system provides several benefits such as providing flangeless chromatography fittings for observing mixtures and solvents. Flangeless Fittings are easy to use. Just slip the nut and ferrule over the tubing and finger tighten the assembly into your receiving port. Our Flangeless Fitting require minimal downtime because component replacement is…
Our Super Flangeless Fittings provide the highest pressure holding capability in a flat-bottom fitting system that we offer. Our unique design eliminates loosening of fittings due to tubing twist and holds tight even through vibration. Our high pressure fittings are excellent for tubing assemblies and those times when connections need to be broken frequently.
Our high pressure actuated valves are designed to be added to your existing instrument or to work optimally with stand-alone lab configurations. Our high pressure valves offer switching, injection, and selection valve functionality ideal for UHPLC applications. These valves have a pressure rating of up to 15000 psi (1035 bar).
Utilize the increased separation speed and resolution of the Thermo Scientific™ Vanquish™ UHPLC system to detect and quantify virtually any substance using the near-universal detection capabilities of Vanquish Charged Aerosol Detectors. Ideal for everyday analyses, the Thermo Scientific™ Vanquish™ Charged Aerosol Detector F offers enhanced linear dynamic range and accurate, consistent response. This detector can measure low-le…
Improve your productivity and sample knowledge for a faster return on investment with the Thermo Scientific™ Vanquish™ Duo UHPLC systems. Vanquish Duo UHPLC systems use a separate second flow path in one integrated system. With the Thermo Scientific™ Vanquish™ Duo UHPLC system for Inverse Gradient, improve your ability to detect and quantify all compounds in your sample with universal Charged Aerosol Detection using an Inverse…
Improve your productivity and sample knowledge for a faster return on investment with the Thermo Scientific™ Vanquish™ Duo UHPLC systems. Vanquish Duo UHPLC systems use a separate second flow path in one integrated system. With the Thermo Scientific™ Vanquish™ Duo UHPLC system for Tandem LC or LC-MS helps you get the most out of your detector or mass spectrometer? The system accomplishes this by running your analysis on two pu…
Combine maximum injection precision and huge sample capacity. The Thermo Scientific™ Vanquish™ Split Samplers employ high-throughput performance for the highest confidence in peak identification and quantitation possible. The samplers provide fully biocompatible fluidics, unsurpassed sample dosing precision, and is optimized for ruggedness and uptime, even under the toughest system pressure of up to 1500 bar (22,000 psi) and c…
Combine maximum injection precision and huge sample capacity. The Thermo Scientific™ Vanquish™ Split Samplers employ high-throughput performance for the highest confidence in peak identification and quantitation possible. The samplers provide fully biocompatible fluidics, unsurpassed sample dosing precision, and is optimized for ruggedness and uptime, even under the toughest system pressure of up to 1500 bar (22,000 psi) and c…
Improve productivity using the Thermo Scientific™ Vanquish™ Dual Split Sampler F. Designed to support Thermo Scientific™ Vanquish™ Duo UHPLC system for Dual LC, this unique biocompatible autosampler with 1000 bar pressure, the Vanquish Dual Split Sample F combines two injection devices in one housing, resulting in two separate channels that are individually controllable (injector volume, wash cycles, etc.).
Improve productivity using the Thermo Scientific™ Vanquish™ Dual Split Sampler H. Designed to support Thermo Scientific™ Vanquish™ Duo UHPLC system for Dual LC, this unique biocompatible autosampler with 1500 bar pressure, the Vanquish Dual Split Sample F combines two injection devices in one housing, resulting in two separate channels that are individually controllable (injector volume, wash cycles, etc.).
Employ an intelligent robotic that is fully integrated into a Thermo Scientific™ Vanquish™ UHPLC system with the Vanquish Charger module. With a capacity of 8832 samples (23 well plates), the Charger module automates sample loading into the Vanquish system for extra-long unattended operation. Environmental controls ensure reliable sample protection. A barcode reader automatically inventories each plate or rack within the devic…
Reduce the size and cost of your liquid chromatography (LC) system. The Thermo Scientific™ UltiMate™ ACC-3000 Autosampler Column Compartments provides a cost-effective solution for conventional LC and UHPLC applications at up to 62MPa (9000 psi) operating pressure. It integrates an autosampler and column compartment (with optional sample thermostatting) in a single module and offers performance and flexibility that exceed mos…
Simplify off-line multidimensional and multistep liquid chromatography (LC) in analytical and micro flow rate ranges. The Thermo Scientific™ UltiMate™ Autosampler with Fraction Collector uniquely supports both sample injection, fractionation, and reinjection using two integrated switching valves. The instrument fully automates multidimensional separations, allowing columns that differ in chemistry and internal diameter to be c…
Extend your biocompatible flow path for sensitive analyses. The Thermo Scientific™ Dionex™ UltiMate™ WPS-3000TBRS Biocompatible Rapid Separation Well Plate Autosampler protects your samples from start to finish with a ceramic needle, titanium injection valve and biocompatible fingertight fittings. This UHPLC autosampler uniquely injects up to 500µL at pressures up to 15,000 psi (103.4 MPa). The in-line split-loop (or flow-thro…
Obtain maximum application flexibility including Dual LC, Tandem LC or LC-MS, and Inverse Gradient workflows using the biocompatible Thermo Scientific™ Vanquish™ Dual Pump F. This pump provides two independent ternary-solvent blending flow streams in one housing. Our built-in Thermo Scientific™ SmartFlow™ pumping technology automatically compensates for changing eluent compressibility ensuring excellent flow and gradient preci…
Enjoy the flexibility of a quaternary pump with the fast gradient performance of a binary pump. The unique Thermo Scientific™ UltiMate™ LPG-3400XRS Extended Pressure Range Quaternary Pump is an LC/MS optimized quaternary UHPLC pump offering state-of-the-art performance for high speed and ultrahigh resolution applications. The compact pump head design, with a gradient delay volume of just 200µL, supports ballistic gradients for…
Extend the capabilities of the Thermo Scientific™ Vanquish™ UHPLC platform by adding all the flexibility you can expect from a state-of-the-art quaternary solvent blending. The biocompatible Thermo Scientific™ Vanquish™ Quaternary Pump F incorporates our SmartFlow™ pumping technology, which ensures excellent flow and gradient precision, independent of eluent composition and backpressures up to 1034 bar. This gives you unrivale…
For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ UltiMate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. With an unrivaled flow-pressure footprint, the RSLC pump is the most flexible binary UHPLC pump available. The UltiMate 3000 Binary RS pumps are capable of providing true ballistic gradients with excellent retention time precision…
With exceptional flow stability and adjustable delay volume, the Thermo Scientific™ UltiMate™ HPG-3200RS Rapid Separation Binary Pump excels at high-speed and high-resolution applications. An industry-leading flow/pressure footprint of 100 MPa (15,000 psi) at up to 5mL/min. and 80 MPa (11,600 psi) at 8mL/min. makes it the most flexible binary pump choice. Designed with a stainless-steel-free (biocompatible) flow path, the HPG-…
Enjoy accurate gradients and precise retention times while meeting the requirements of diverse sample types with a wide range of flow rates. The Thermo Scientific™ UltiMate™ HPG-3200SD Standard Binary Pump is ideal for analytical LC separations on standard-bore columns. With support for pressures up to 62MPa (9,000 psi, 620 bar) at flow rates of up to 10 mL/min., UltiMate 3000 SD pumps excel in standard LC applications offer a…
Developed to offer more pressure capabilities than ever before, the Thermo Scientific™ Vanquish™ Binary Pump H comes without any tradeoff on durability and robustness. From ultrafast to extremely shallow gradients at pressures up to 150 MPa, the industry-leading fourth generation Thermo Scientific™ SmartFlow™ pumping technology of the Vanquish system always provides you with unmatched retention time reproducibility and the low…
