High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phas…
The 1260 Infinity II High-Temperature GPC System can provide information about all of today’s engineering polymers such as polyolefins, which are one of the most popular polymers with numerous applications. This level of detail helps with development and to predict how a given batch will process and perform in the final product.
By combining the signals from two diode array detectors with different path length Max-Light flow cells, the 1290 Infinity II High Dynamic Range DAD Solution allows you to detect and quantify all components in your sample in a single run. The 30 times wider linear UV range offered by the high dynamic range detection expands your UV detection capacity. The system is ideal for analysis of mixtures with widely different concentr…
CoulArray® - The Only Coulometric Array Detector for HPLC ESA's pioneering electrochemical technology has made possible the multi-sensor detector for HPLC. Measure multiple analytes simultaneously, including those that are chromatographically unresolved. Only ESA's unique coulometric array detection provides: Sensitivity to femtomole levels 3D resolution of co-eluting peaks Qualitative characterization of peaks Simpl…
ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…
The Finnigan Surveyor Plus family of HPLC instruments is specifically optimized for the most demanding HPLC and LC/MS applications. Short cycle times, high sample capacity, low flow rate performance, and patented LightPipeTM technology have been integrated into one complete system for high-throughput sample processing environments.
The EXP® Guard Column cartridge system is rated to 15,000 psi with hand-tight replaceable cartridges. This unique design connects directly to any UHPLC column with the auto-adjusting Titanium Hybrid Ferrule and EXP® Fittings, providing repeated zero-dead-volume connections and easy adjustability between various column types. Free-Turn¨ architecture allows the user to change cartridges by hand without breaking fluid connections…
The Agilent open-systems approach to laboratory informatics allows our customers to select the best hardware and software to meet their needs. The instrument control program consists of two components to enable multivendor control in chromatography data systems (CDS). Instrument control framework (ICF) and RapidControl.Net (RC.Net) standardize how chromatography data systems (CDS) interact with analytical instruments.…
The Shim-pack XR-ODS, which was developed with the aim of increasing the speed attained under standard column pressure conditions, has been joined by the Shim-pack XR-ODS II, which was developed in order to facilitate high-speed, high-separation analysis for a wider range of applications, such as those involving methanol mobile phases, by using a new long column. The Shim-pack XR-ODS II is a high-separation analysis column tha…
Adding a syringe filtration step prior to injection not only helps to ensure more consistent and reliable results; it also helps protect delicate instruments and prolongs column life. Available in sizes and membrane types to meet any application need, Thermo Scientific™ Titan3™ Syringe Filters provide cleaner sample extracts by removing interfering materials and fine particles down to the rated pore size. Titan3 Nylon Syringe…
