High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The OPTI-GUARD® 3 mm Columns maintains the tool-free connectivity of the OPTI-GUARD 1 mm, but incorporates a cartridge based format to allow for a larger bed with more capacity. The two-part holder is designed for use with any analytical column to provide vital protection from dirty samples and strongly retained contaminants. An auto-adjusting stem conforms to any tube stop depth for a perfect zero-dead-volume connections.Key…
OPTI-SEAL® Piston/Plunger Seals are the longest lasting seals on the market. Machined from either of two proprietary polymer blends, UHMW-PE or ITB, Optimize Seals provide the ultimate in high pressure sealing capacity. For mostly aqueous buffered mobile phases, OPTI-SEAL® UHMW-polyethylene is the wisest choice. Specially designed to be more resistant to abrasion and wear than standard polyethylene seals, they can last up to s…
Optimize realizes that your time is valuable and should not be wasted worrying about fittings. For this reason, all Opti-Lock Fittings are precision machined in the USA for optimal quality and thread consistency. The machining process is the best way to ensure tight manufacturing tolerances and minimal product variance. What does all this mean? It means that every fitting you purchase from Optimize will perform just as well as…
Optimize Back Pressure Regulators utilize an active mechanism to maintain steady back pressure across a range of mobile phase viscosities and flow rates. Placing one downstream from your detector will prevent solvent decompression and bubble formation in the flow cell, reducing baseline noise and drift. Each pre-set Optimize stainless steel Back Pressure Regulator offers a 100µL internal volume and includes fittings for immedi…
Optimize Replacement Pistons/Plungers are, by design, a consumable part of the HPLC pump and should be replaced on a regular preventative maintenance schedule. To maintain peak performance and avoid unplanned downtime, sapphire pistons require inspection every six months with yearly or as-needed replacement. Optimize sapphire pistons offer optimal concentricity and resistance to wear. Each sapphire rod is specifically cut in r…
The OPTI-LYNX™ Quick Connect Filters allow you to connect any 10-32 valve/port for trapping or analytical column for pre-column filtration. With OPTI-LYNX™, accessing and changing the insert takes only a quarter turn. Replace traps, filters or change configurations in seconds, without tools. OPTI-LYNX™ traps and filters (ordered separately) come in several bed volume/ diameter/ porosity combinations, allowing you to find your…
The OPTI-LYNX™ Quick Connect Trap Columns allow you to quick-connect to any 10-32 valve/port for trapping or analytical column for pre-column filtration. With OPTI-LYNX™, accessing and changing the insert takes only a quarter turn. Replace traps, filters or change configurations in seconds, without tools. OPTI-LYNX™ traps and filters (ordered separately) come in several bed volume/ diameter/ porosity combinations, allowing you…
The OPTI-LYNX™ QuickConnect Guard Column is a high-performance, cartridge-based guard system, suitable for use with any 10-32 analytical column due to its auto-adjusting ZDV port adapter. OPTI-LYNX™ combines effective column protection with the convenience of quick-connect operation. Some cartridge-based guard column products offer convenience with a price: higher internal volumes. Optimize Technologies, refuse to compromise p…
High-efficiency separations at any pH The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (> 100°C), does not shrink or swell, and is completely inert to most conditions commonly encountered in reversed-phase chromatography. The PRP-C18 is…
Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence.
ZORBAX RRHD 300-HILIC columns for glycopeptide analysis feature 1.8 µm particles and a 300Å pore size. These enable you to resolve peptide glycoforms.
LyoSpeed™ delivers exceptional results with even the most difficult samples. When working with certain solvents, e.g. HPLC solvents, a fully dried result can be hard to achieve due to interactions with the dissolved sample - resulting in the formation of a gum or oil.In response to customer demand Genevac have developed a Fast Lyophilisation, or LyoSpeed™ process to resolve these problems for customers working with HPLC solve…
LiChrospher silica columns are made with spherical, 'sil' type porous silica particles. LiChrospher Columns Features: Available in 5 µm particle size Reversed-phase available in RP-C18, RP-18 Encapped, RP-8, RP-Select B, CN Normal-phase available in CN, Diol, NH2, Si Applications—Pharmaceuticals, aromatics LiChrospher RP-18—Spherical silica for acidic, neutral and basic compounds with great batch-to-batch reproduci…
The ZORBAX NH2 can be utilized for normal-phase and weak anion-exchange compounds and can separate vitamins, carbohydrates and sugars in the reversed-phase mode. The NH2 consists of a propyl-amino silane phase bonded to ZORBAX SIL. For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings. ZORBAX NH2 Features: Amino-propyl silane phase bonded to ZORBAX SIL Used for…
The ZORBAX RX-SIL is made from very pure porous silica microspheres and is useful for very hydrophilic compounds with high organic mobile phases. With low acidity and metal content the ZORBAX Rx-SIL is ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica. The ZORBAX Rx-SIL is also stronger than other silica types and is less acidic than the ZORBAX-SIL. ZORBAX Rx-SIL Featu…
Polymer bulk packing material for anion exchange applications. All Hamilton HPLC packings are available as bulk resin or packed into analytical, semi-preparative and preparative columns. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method/process validation. For ease of ordering specify the quanti…
Eshmuno® A is a rigid, high capacity Protein A affinity chromatography resin for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies. Eshmuno® A resin can be cleaned and sanitized under acid and alkaline conditions while maintaining high dynamic binding capacity at high flow rates.The Eshmuno® A resin contains a EMD Millipore proprietary ligand. This ligand is recombinantely expresse…
The PDS-100 is a Programmable Dispensing System which uses precision stepper motors to control FMI “Q” and “RH” pump models including STQP, STQ, STH, & STRH in all piston sizes. The FMI pumps are integrally mounted to the programmable controller. The controller contains the stepper motors and driver electronics, and is a rugged anodized aluminum design. Several single and dual pump configurations are available including a dua…
PiP Pumps micro π-petter - Pipetting, Syringing and Diluting Ideal for repetitive and volumetric dispensing of acids, solvents and aqueous solutions. Model "PiP" Precision Dilutor Features: FMI's unique low dead-volume pump heads, and synchronous motor drives. Can act as a single shot dispenser using the hand/foot switch or as a single metering pump in the continuous mode. Using a combination of forwar…
Ideal for Analyzers, Environmental Sampling & Injection, & Low Current Remote Battery Operation Model QBG Low Current DC Features: Ideal for extended 12/24 volt battery operation in remote locations. They are rated 60 RPM at 12 VDC and 120 RPM at 24 VDC. One Moving Part - NO VALVES! Low Current DC Operation Self Priming Patented CeramPump® Design
With a choice of over 40 different phases (and we continue to develop additional, unique and powerful HPLC sorbents) our SiliaChrom series of HPLC columns surely has the reversed or normal phase you need for your application.Mostly made from a silica-based sorbent, we carefully monitor the manufacturing process, from raw material synthesis to packing, ensuring you that our HPLC columns are of the highest quality.All columns ar…
For two decades, SiliCycle has been dedicated to silica gel manufacturing and has achieved a strong know-how and recognized expertise in this field. Furthermore, to support the increasing demand of our spherical silica gels, we have developed and optimized a highly controlled, large-scale, production process for all our SiliaSphere products, without compromising silica quality. SiliaSphere silica gels are manufactured from a…
SpeedCore is the very latest in core-shell technology for use in HPLC and UHPLC. Designed to offer enhanced efficiency, speed and resolution of analysis. Speedcore C18 is designed to provide characteristics which will enhance method development. It provides the ability to obtain sharp peak shapes whilst retaining and separating a wide variety of compounds both hydrophobic and hydrophilic.SpeedCore C18 Features: C18 Hydroph…
HT4000L is an HPLC autosampler for high presise injection into HPLC, UHPLC, LC/MS and IC systems. HT4000L is lab automation ready. In fact HT4000L besides working as a standard HPLC autosampler, can take care of many sample preparation tasks as no standard autosampler has been able to assist up to now. Basic sample preparation capabilities includes dilution, precolumn derivatization, pH spiking and internal standard addition…
HT310L is a cost-effective HPLC autosampler, that offers automation for processing up to 10 unattended samples. By its competitive pricing is the alternative to the manual injector for users that cares about reproducibility and accuracy. HT310L fits every HPLC system, and its direct syringe injection minimizes dead volume, reduces carry-over and improves repeatability. Full and partial loop are both performed with very accura…
