High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The SEDEX Model 75 provides the option of 4 new nebulizers and a redesigned evaporation tube. These changes allow you to use a lower temperature to evaporate the mobile phase, this provides a more uniform droplet size, maximizing the sensitivity and repeatability. Enhanced ease of operation The SEDEX Model 75 is designed to be very easy to use. The operator needs to set only three controls, the gain, the zero and the evaporati…
4.6mm, 4.0mm, 2.0mm, and 1.0mm ID HPLC columns are available in lengths of 50mm, 100mm, 150mm, and 250mm. SGE has biocompatible GLT columns available for applications requiring truly inert HPLC columns. SGE HPLC columns offer brilliant results for reverse phase, and normal phase HPLC applications.
The LC-4000 Series includes three PDA detectors.The MD - 4 017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhor se for UV- visible detection in the range 200 to 600nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution. The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detec tor. Making use of a 1024 eleme…
The FP-4020 fluorescence detector has been designed for the pursuit of extreme sensitivity.Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperature- regulated cell body has led to the highest sensitivity with stable detection. The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a gi…
The OR-4090 Chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption.The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV/Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of…
Visco Mixers are static mixers for HPLC and other high pressure applications. The Lee Visco-Jet Micro-Mixer uses aerospace technology to provide the ultimate in static mixing efficiency. A series of 36 critically controlled spin chambers subject the incoming liquids to a vigorously repeated mixing process. No electrical or mechanical input is required – the mixing energy is drawn from the liquids themselves. Two sizes ar…
To successfully separate trityl-off, deprotected oligonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions.
Detection to trace levels of some metabolites, such as histamine, some amino acids, some steroids and catecholamines, is quickly and simply achieved, with Cecil Electrochemical Detection Adept HPLC.
Stainless steel fittings remain the most popular fitting used on standard HPLC systems today, due to their high temperature resistance and high-pressure holding abilities. Our durable and strong 316 Stainless Steel Fittings are rated to 20,000 psi (1,380 bar) when wrench tightened. You can choose our Standard Fittings, or select from other manufacturer-compatible offerings.
MX Series II™ actuated valves are designed to be added to your existing instrument or to work optimally with stand-alone lab configurations. Our valves can be controlled remotely or operated manually using the push-button front panel with LED position indicator. They easily connect to your instrument or PC through contact closure, BCD, serial port, or USB. Commands can be sent to the MX valves using your chromatograph…
We offer an innovative line of Very High Pressure (VHP) fittings, designed to withstand extreme pressures. This patent-pending line of ground-breaking fitting systems is perfect for use within the increasingly demanding requirements of today’s UHPLC high performance analytical systems.
The Ascentis Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 ?m particles while maintaining lower backp…
Polymer-based C18 columns for Reversed Phase Chromatography.
Silica-based columns for aqueous Size Exclusion Chromatography (GFC).
