High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Spark Holland has been operating at the forefront of autosampler design for 25 years. Spark’s Reliance™ autosampler is the first true bio-injector, and is preferred by customers for 4 simple reasons: - Lowest possible carry-over- High-speed wash cycles- Low maintenance- Conditioned sample storage The Reliance™ autosampler complies with GLP requirements and can be coupled with most LC-MS systems. It runs fully integra…
Hypersil Green columns allows for sharp, symmetrical and easily quantifiable assays to be developed quickly and easily. Product detail: Hypersil Green columns are developed for environmental analysis. The chromatographic separation of phenols, nitrophenols, chlorophenols, triazines, urea pesticides, phthalates and many other polar compounds requires a separating phase specially designed for the task. Hypersil Green ENV has…
o The benchmark in based deactivated silica columnso Sharp peaks and improved symmetry for basic compoundso Value and performance from a globally referenced column Product detail: DASH columns are packed to the same high standards as our analytical columns, and offer superioir chromatographic resolution in a design that eliminates the need for endfittings and wrenches during column change over. This makes column inst…
Fluophase RP Columns o A range of perfluorinated phases for alternative selectivityo Extra retention and selectivity for halogenated compoundso Select from 100Å and 300Å pore sizes Product detail:For alternative selectivity to traditional alkyl phases, we recommend you try the perfluorinated phases of Fluophase columns. The stationary phase of Fluophase RP contains a perfluorinated C6 chain, where every hydrogen atom on…
Hypersil 100 C8 Columns Compared to other classical materials, Hypersil 100 has a smaller pore diameter of only 100Å, which creates a higher specific surface area that can be advantageous where longer retention times are needed, such as chromatography of polar compounds. Product detail: Compared to other classical materials, Hypersil 100 has a smaller pore diameter of only 100Å, which creates a higher specific surface area…
The UNIGUARD drop-in cartridge fits both our guard systems: the stand alone UNIGUARD and the Direct Connection UNIGUARD. Product detail: We recommend all analytical columns are protected by a guard cartridge, which is replaced on a regular basis. The ID of the analytical column determines the correct ID for the guard cartridge. We offer 10x1.0, 10x2.1, 10x3.0, 10x4.0 (for use with both 4.0 and 4.6mm ID analytical columns) i…
PRP-X600 is a superficially porous weak-base anion exchange support that separates DNA oligomers according to negative charge. PRP-X600 provides the following benefits:Fast SeparationThe unique porosity provides fast separation with better sample capacity than non-porous supports.StabilityThe polymeric PRP-X600 support is stable to a variety of mobile phases. From inorganic buffer solvent to high concentration organic modifier…
The SPECTRUM range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, in Discovery and Development, who are looking purify samples from mgs to 5-10 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…
The Shimadzu Prominence high-performance liquid chromatograph achieves an exceptional level of performance, reliability and great expandability to support diverse applications from ultra-fast liquid chromatography to preparative LC, gel permeation chromatography (GPC), ion chromatography, and LC/MS.Many analysis techniques use LCMS, which requires a front-end HPLC system to provide solvent delivery performance in the micro to…
The Prominence UFLC Series is an ultra-high-speed LC that achieves both ultra-high-speed analysis and ultra-high separation, based on high analysis precision and reliability. In addition to shortening analysis times, thereby heightening analysis efficiency and conserving solvent, this instrument supports reliable separation and detection of trace materials in a variety of fields. Applications include the evaluation of trace re…
