Elisa
The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity t…
The CHEMICON ssDNA Apoptosis ELISA Kit is a convenient, sensitive method for early detection of apoptosis. The assay can be performed in two formats: 1. Cells are grown, treated with apoptosis inducing agents, stained and analyzed in the same microtiter plate. This assay is suitable for high throughput screening when induction of apoptosis is used as the endpoint of drug activity. 2. Cell suspensions obtained from cultures or…
The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.
The human C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures human CRP.
With the ChemiKine assay system, sheep polyclonal antibodies generated against human NT-3 are coated onto a microplate and are used to capture NT-3 from a sample. NT-3 specific, biotin conjugated, mouse monoclonal antibodies detect the captured NT-3. After addition of streptavidin-enzyme, substrate and stop solution the amount of NT-3 is determined. The standard curve demonstrates a direct relationship between Optical Density…
With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample. ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay…
ChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat. ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay…
ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin. With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects…
The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference.
The determination of levels of extracellular matrix molecules is becoming more important to understanding alterations in matrix function during development and tumorigenesis. Questions of matrix gene regulation and function require a sensitive means of determining the levels of protein expression by cells.
