Lightning-Link™ Atto 532 kit

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™!

• Eliminates need for secondary antibodies
• Reduces the number of wash steps
• Ideal for multi-protein analysis – with over 40 available labels
• Very easy to use
• 100% antibody recovery

Lightning-Link™ kits

The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.

Go ‘direct’

Direct antibodies labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money.

The antibody conjugate:

Atto532 is one of a new generation of fluorescent labels. Atto532 belongs to the Rhodamine class of fluorescent dyes. It has a strong absorption at 532nm, high fluorescence at 553nm (extinction coefficient 1.15 x105 cm-1M-1) and quantum yield. Atto532 can be used as a substitute dye for Alexa Fluor 532, Erythrosin, BODIPY R6G, 6-Hexachlorofluorescein (HEX), BODPY-TMR-X, and 5(6)-Carboxytetramethylrhodamine (TAMRA).