High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Designed for Rapid Resolution and Ultra Performance LC Systems • Communicates with ChemStation via an exclusive SofTA Driver. • Superior HPLC Detection to Replace or Complement your Current Detection. • Sensitive Detection for a Wide Variety of Chemical Classes and Structures. • Easy to Use, Fast Start-up, No Consumable Parts, Low Cost of Operation. • Precise Vapor Phase Control with SofTA Exclusive Thermo-Split™ Technology. •…
Biochrom instruments are supplied complete with the analytical column of your choice.Manufactured from PEEK material, the columns are free from corrosion and metal contamination and are packed with optimally sized, cation-exchange resin. The columns are attached with finger-tight fittings so no special spanners are required to ensure a leak-free seal. Additional analytical and prewash columns can be purchased. The Biochrom…
Specially designed for use with the Cecil Adept HPLC, Q-Adept HPLC, Merit HPLC and IonQuest ion chromatography.
A single quaternary low pressure mixing analytical pump, used in Q-Adept HPLC modular systems
A range of 0.01 to 50 ml/minute, used to create a high pressure mixing gradient.
The Accela 600 LC pump offers a maximum operating pressure of 600 bar and involves the use of unique and innovative Force Feedback Control (FFC), enabling the delivery of accurate and precise flow and gradients under all operating conditions. This pump is able to deliver unmatched compositional and flow rate accuracy and precision over the entire operating range resulting in exceptional reproducibility. Designed for configurat…
The Accela 1000 LC pump offers a maximum operating pressure of 15,000 psi and involves the use of unique and innovative Force Feedback Control (FFC), enabling the delivery of accurate and precise flow and gradients under all operating conditions. This pump is also able to deliver unmatched compositional and flow rate accuracy and precision over the entire operating range resulting in exceptional reproducibility making it an un…
A successful packing material from the start: LiChrosorb® is one of the most successful and reliable packing materials used in HPLC for more than 25 years and documented in literature in the form of several thousand applications. The totally porous irregular particles are finely classified in the 5, 7 and 10 µm range. LiChrosorb® offers the complete programme of non-polar derivatives (RP-8, RP-18, RP-select B) polar derivativ…
Hitachi’s L-2300 Column Oven incorporates new technology that provides superior temperature stability and reduces thermal gradients resulting in greatly enhanced performance. Peltier heating and cooling allow short equilibration times. In addition, solvent is preheated prior to reaching the column which results in stable temperature inside the column. System connectivity ensures that sample injections are made only when temper…
Spherical silica carrier for constant results on high level: LiChrospher® are reliable and versatile traditionally produced spherical silica carrier. LiChrospher® silica carrier are available with different modifications. For the LiChrospher® RP-phases a selectivity and performance guarantee makes sure that these parameters remain constant form batch to batch and from year to year. The polar modified phases LiChrospher® CN, L…
Hitachi's LaChrom Elite HPLC and Sensivate Elite PCR deliver turn-key analysis kits for a variety of compounds. Carbamate pesticides and glyphosate herbicide kits are available, which achieve low-level detection with high selectivity. Derivatization kits with ninhydrin or o-phthaldehyde offer cost effective solutions for the analysis of Amino Acids via PCR. The kits include analytical columns, reagents, buffers, and instructio…
LiChrospher® CN is a versatile HPLC sorbent based on spherical silica particles possessing both polar and hydrophobic properties. LiChrospher® CN may be used as weakly hydrophobic sorbent in reversed phase chromatography or as polar sorbent in normal phase chromatography.
LiChrospher® DIOL is a versatile HPLC sorbent based on spherical silica particles possessing both polar and hydrophobic properties. LiChrospher® DIOL may be used as weakly hydrophobic sorbent in reversed phase chromatography with some limitations or as polar sorbent in normal phase chromatography. LiChrospher® DIOL is also suitable for exclusion chromatography.
A versatile sorbent for both reversed phase and straight phase chromatography: LiChrospher® NH2 is a versatile HPLC sorbent based on spherical silica particles possessing both polar and hydrophobic properties. LiChrospher® NH2 may be used for normal phase chromatography, reversed phase chromatography and for ion exchange chromatography. Typical applications are the separation of carbohydrates (mono-, di- and oilgosaccharides)…
A new member of the HyPURITY® family introduced at Pittcon 2003, HyPURITY AQUASTAR™ columns are a new silica-based polar end-capped C18 which is designed to offer superior retention of polar compounds and increased sensitivity in 100% aqueous conditions. The robust nature of the bonded phase results in a silica-based column that is unsurpassed for LC and LC/MS applications. This next generation column also offers enhanced perf…
LiChrospher® PAH is a highly efficient and selective HPLC column especially designed for the high resolution separation of 16 PAH (polycyclic aromatic hydrocarbons) according to EPA 610 and 550 + benzo(e)pyrene + perylene. LiChrospher® PAH is based on a special modified RP-18 silica gel to achieve highly resolved PAH separations. LiChrospher® PAH can be used for both, the isocratic separation of 6 PAH according to the German…
For reproducible reversed phase separation: LiChrospher® RP-phases are reliable and versatile traditionally produced spherical silica carrier with reversed phase properties. They are well suited for the chromatography of acidic, neutral and weakly basic compounds, substances found frequently in all analytical fields. A selectivity and performance guarantee makes sure that these parameters remain constant form batch to batch a…
Excellent separations even with basic compounds: LiChrospher® RP-select B is a versatile reversed phase sorbent based on spherical silica particles with excellent properties for the determination of basic substances, but with still good properties for the determination neutral and acidic substances. LiChrospher® RP-select B is optimised in order to prevent any secondary interactions with basic substances and guarantees that…
High-resolution separations of peptides and tRNA molecules: LiChrospher® WP 300 RP-18 is a highly selective and reliable HPLC column for the separation of peptides and low molecular weight proteins. LiChrospher® WP 300 RP-18 enables the tailing-free separation of basic compounds and is excellently suited for the separation of tRNA molecules. High recovery rates are achieved, especially in the case of highly hydrophobic pepti…
The GPC Quattro is designed for the clean-up of complex animal tissues, food products and environmental samples by separating high molecular weight matrixes such as lipids, carbohydrates, pigments, etc., from analytical samples. Beneficial features of the GPC Quattro include: • Fourfold increase in productivity in comparison to traditional single-column instruments• High throughput • Economical price • Full error management…
UHPLC quaternary solvent delivery system with operating pressure to 1250 barThe Thermo Scientific Accela high-speed chromatographic system provides fast, efficient HPLC and U-HPLC in One Efficient, Limitless System. The Accela™ 1250 High Speed LC pump provides accurate and precise flow and gradients under all operating conditions with minimum pulsation. Features of the UHPLC Accela 1250 pump: • Quaternary capabilities with f…
The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations: • Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons • Non-circular drive gears provide smooth, uniform eluent delivery • User-programmable flow rate calibration and compressibility compensation factors ensure straightforward pump operation qualification • Tests t…
Allows simpler, time saving methods for reversed phase chromatography of basic compounds: Purospher® RP-18 is a versatile HPLC column designed for the separation of problematic basic compounds with simple neutral eluents. This results in less time and cost because of simpler method optimization. Purospher® RP-18 is based upon a high purity, metal free silica. The multi-step chemical modification and deactivation of the surface…
Excellent peak symmetry with either basic or strongly acidic compounds: Purospher® RP-18 endcapped is a versatile HPLC column producing excellent peak shapes, designed for both the separation of basic compounds with simple neutral eluents and for the elution of strongly acidic compounds. Excellent separations in a shorter period of time with very good peak symmetry saves time and money. The excellent balance of Purospher® RP-1…
