Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The ExAssist helper phage with XOLR works with the Lambda expression vector system kits for mass excision of libraries in ZAP Express or Lambda ZAP vectors. The pCMV-Script or pBluescript phagemid will grow.
Agilent provides a range of Interference-resistant Helper Phages that enable ssDNA recovery from phagemid vectors. The pBlueScript II Phagemid Vector systems are available in R408 and VCSM13 format. R408 Interference-Resistant Helper Phages are stable and easy to grow, and are provided in a single-strand size of ~4 KB.
Helper phages for mass excision of Lambda ZAP, ZAP Express libraries, and for single strand rescue protocols. Use for mass excision of Lambda ZAP®, and ZAP Express® libraries Interference-resistant helper phage Eliminates the possibility of co-infection from helper phage
Agilent miRNA qPCR Detection Reagents are high-specificity reagents for the polyadenylation of microRNAs (miRNAs) from total RNA. The miRNA qPCR Detection Reagents also enable the synthesis of first-strand cDNA from the tailed miRNAs.
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. New cDNA synt…
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. CMV promoter for high-level protein expression in mammalian cells Large multiple cloning site contains 15 unique sites Clone PCR products generated with any PCR e…
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods.
The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells. In situ staining of cells directly in dishes without removing or manipulating cells Rapid detection of Ÿ-gal activity in transfected cells Results in 24 hours Maximum signal with low background Deep blue staining reaction Kit in…
