Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…
APAgene™ GOLD Genome Walking Kits are designed to rapidly and reliably amplify unknown genomic DNA using our patented APA technology: 1. Isolate unknown genomic sequences flanking transgenes (including T-DNAs, gene traps, transposons), sequence-tagged sites (STSs) or expressed sequence tags (ESTs). 2. Isolate localized sequences flanking known sequences from large clones and genomic DNAs. 3. Sequence insert ends of large clone…
Improve your cell analysis and use our powerful new transfection reagents: Efficiently transfect a broad spectrum of cells, including difficult-to-transfect cell lines and tumor cell lines. Generate physiologically relevant results using low cytotoxic reagents. Avoid time-consuming optimization using fast, reliable protocols.
OneTRAC™ allows you to profile every gene, validate biomarker signatures, and apply your findings across all your studies. One-stop service for all your gene expression profiling needs All-inclusive gene expression data for pattern recognition and pathway analyses at the macro- and micro-levels Streamlined and integrated methodology from screening to validation of gene signatures Cost-efficient expression profiling f…
GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Whereas many available transfection reagents…
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Based on the T7 promoter-driven system, Novagen’s pET System has been used to express thousands of different proteins. pET Vectors are supplied as purified plasmid DNA (10 µg) plus an Induction Control strain (supplied as a glycerol stock). Complete sequences and detailed vector maps are available.
Powerful transfection reagent, based on an innovative biochemical method. Next generation of outstanding transfection reagent, highly efficient, universal and multipurpose.
For all nucleic acids and all transfection conditions.The universal and powerful PolyMag formulation allows achieving very high transfection efficiency. It is composed by magnetic nanoparticles coated with cationic molecules. Exclusive and optimized PolyMag reagent has been successfully tested with:- All nucleic acids types: naked DNA, RNA, siRNA, ODN, etc…- Cell lines, primary and hard to transfect cells- Adherent and suspens…
CombiMag improves your transfection reagent efficiencyCombiMag reagent has been designed to be employed in association with all transfection reagents complexed with any types of nucleic acids. All cell types, even hard-to-transfect and primary cells, can be transfected using CombiMag associated to any working reagent, even if its original efficiency is low.CombiMag has been associated with many existing transfection reagents i…
FlyFectin ™ is a specifically designed and very efficient transfection reagent for insect cells. Innovative and unique, FlyFectin ™ is: • Highly efficient with excellent reproducibility• Multipurpose (various types of nucleic acids)• Ideal for recombinant protein production with Baculovirus expression system• Simple, Ready-to-use and Rapid• Compatible with and without serum-containing culture media• Non toxic• Economi…
