Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance using Applied Biosystems TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
TaqMan® PreAmp Pools are pre-pooled sets of Applied Biosystems TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are availab…
The 2X Multiplex Master Mix, optimized for the Multiplex PCR using Hot-start Taq., is a Pre-Mix product effective in amplifying a number of DNAs with just a one-time PCR. Key features include: 1. Optimization for Multiplex PCR 2. Contains non-specific outputs 3. Enhanced user convenience 4. Applicable in various tests
All water products are certified to be nuclease free. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC; available in 100 ml, 500 ml and 1 liter sizes. Ambion also offers nuclease-free water that has not been DEPC treated for applications that may be negatively affected by residual DEPC (e.g., oocyte injection). Our nuclease-free, non-DEPC treated water is available in 500 ml…
GenomeWalker™ Kits provide a simple, PCR-based method for walking upstream or downstream in genomic DNA from a known sequence, such as an expressed sequence tag (EST). GenomeWalker allows you to take individual steps of up to 6 kb in genomic DNA using long-distance PCR. Walks can be extended simply by taking multiple steps using new primers based on the sequence obtained in previous steps.
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Transcription Factors and related genes reformatted onto an OpenArray plate. Kit includes: Open…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Inflammation and related genes reformatted onto an OpenArray plate. Kit includes: OpenArr…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Transcription Factors and related genes reformatted onto an OpenArray plate. Kit includes…
LightCycler® 1536 Real-Time PCR System from Roche Applied Science - High Throughput - Redefined Forge ahead into a new era in high-throughput real-time PCR with the new LightCycler® 1536 RT-PCR Instrument. Precisely engineered for miniaturization and parallelization, this latest innovation reinforces Roche Applied Science as a global expert in real-time PCR. Take advantage of over ten years of publication-proven expertise with…
Finnzymes’ novel approach, Direct PCR, saves you time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Finnzymes' Phusion® High-Fidelity DNA Polymerases and Phire® Hot Start DNA Polymerase. These unique fusion enzymes have been engineered to incorporate a double-stranded DNA binding domain that gives them extraordinary properties. Phusion and Phire DNA Polymerases are except…
For amplification of DNA directly from plant material. Finnzymes’ Phire® Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA isolation is required prior to the PCR. The kit is based on Phire® Hot Start DNA Polymerase, a specially engineered enzyme with a unique DNA binding domain. Phire DNA Polymerase is extremely robust and tolerant of many PCR inhibitors present in plant material. The kit incl…
Finnzymes’ Piko® Plate Illuminator is a state-of-the-art plate rack that provides a simple way to track the loading of reactants by illuminating the target well(s) from below with white LED lights. With 10 different pre-programmed loading patterns, this device can fulfill just about any protocol requirements. It accepts both 24-well and 96-well Piko® PCR Plates and can be used with all standard single channel, 8-channel or 1…
The Spartan DX-12™ is designed for non-batched PCR applications. It is a lightweight, stand-alone instrument. • 12-well sample capacity and dual-channel detection • Performs 2 or 3-temperature PCR programs • Small footprint maximizes bench space • Built-in Reverse Transcription (RT) program for RNA analysis • Compatible with leading reagents and kits
96-Well PCR® Rack - Removable hinged lid lies flat The Heathrow Scientific 96-Well PCR® Rack is perfect for pre- or post-PCR sampling. The sturdy polypropylene 96-Well PCR® Rack accommodates individual 0.2 mL tubes or strips of 8 or 12 tubes. Tube wells are easily identified with embossed numbers and letters. The 96-Well PCR® Rack is available in natural, single colors, or assorted colors (one each of blue, green, pink, yellow…
Reversible PCR® Rack - Removable hinged lid fits both sides Flip this polypropylene Reversible PCR® Rack to the size of tubes you need. The PCR side of the rack has 168 wells that hold 0.2 mL tubes. The wells accommodate 8- or 12-tube strips or individual tubes. The opposite side of the Reversible PCR® Rack has 40 wells that hold 0.5 mL tubes. Both sides of the rack have 12 wells for 1.5 mL tubes. Autoclavable. Available from…
Finnzymes’ Piko® Thermal Cyclers represent a fundamental improvement in PCR technology. Piko Thermal Cyclers incorporate novel technical solutions that allow significant reductions in PCR run times and the overall size of the instrument. Although merely half the size of other thermal cyclers, Piko Thermal Cyclers meet the highest criteria in performance expected from a thermal cycler and yet are able to complete a PCR protocol…
Bioneer’s AccuPower® PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer. AccuPower PreMix PCR master mix includes our super-processive “three times faster than Taq” Top DNA Polymerase for the fastest nucleic acid amplification. While Top DNA Polymerase is ideal for applications where you would normally use Taq, if your needs include…
AccuPower® HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart PCR master mix.Bioneer uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Bioneer’s Top DNA Polymerase is inhibited at lower temperatures (
AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized PCR Master Mix of CycleScript Reverse Transcriptase, a primer, and all of the other components for cDNA synthesis conveniently packaged in individual tubes. Simply add template RNA, and water. Then, the reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transc…
The AccuPower® RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal c…
AccuPower® RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low…
dNTP's from Bioneer are cost effective and hightly pure. Bioneer is one of the few manufacturers that actually produces our own phosphoramidites. Our dNTP's are made at our state-of-the-art manufacturing facilities, and Bioneer controls quality every step of the way in order to provide you with the best value for your research dollar.
Bioneer provides the ideal tools for your specialized applications. With the advent of technologies such as real-time detection and quantification, as well as mass-scale genotyping projects, more and more scientists require the use of modified oligos. All common Oligo modifications are available. Our fluorogenic oligos for qPCR, are unsurpassed in quality, and priced for value. In addition to the modifications listed in our or…
Amplify your performance To ensure your instrument is operating consistently at peak performance, we have developed service programs and options to meet your needs. Enjoy peace of mind with Eppendorf Performance Plans and Services for your Mastercycler pro, Mastercycler ep and Mastercycler family thermal cyclers. Performance Plans Features 1. Eppendorf service quality and integrity 2. Certified reports 3. Customized service op…
Handy when using with thermal cyclers A time-saver for anyone working with individual 0.2 mL tubes or strips of 8 or 12 tubes. Moving the 0.2 mL tubes to the thermal cycler is super-simple with this tray—just use handles to lift and carry. Transfer tray is a thin strip of plastic designed to rest perfectly over a 96-well tube rack. Made out of high-impact polypropylene. Available in natural or assorted colors: blue, green, pur…
