Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The TaqMan Custom Plating service offers the convenience of pre-plated TaqMan® Gene Expression Assays, Custom Assays, and Custom Probe/Primer Sets in 96- or 384-well plates. This cost-effective custom plating service gives you the flexibility and reliability you need for your screening applications. Whether you are looking for biomarkers, testing for toxicity, searching for microbes, or performing other high-throughput scree…
Applied Biosystems TaqMan Drug Metabolism Genotyping Assays are available for basic research and clinical research. Genotype over 2,600 high value polymorphisms located in regulatory elements and coding regions for 220 drug metabolism and transporter genes with TaqMan® Assay technology.
TaqMan® Express Plates are a cost-effective and convenient 96-well format complimenting Applied Biosystem's line of individual TaqMan® Gene Expression Assays, and pre-spotted 384-well TaqMan® Arrays. Customize any of the six available plate formats with dried-down TaqMan® Assays by positioning each in specific well locations. This flexibility enables an efficient means to screen biological samples for the presence and amount…
TaqMan® Gene Sets are pre-defined lists of genes grouped by pathway or disease target class which can be ordered separately, as a custom plate, or configured directly onto TaqMan® Arrays to enable you to perform hundreds of real-time PCR reactions simultaneously on the Applied Biosystems 7900HT Fast Real-Time PCR System. Simple to implement, TaqMan Gene Sets are customizable, and when placed on a TaqMan Array, require mini…
TaqMan® Gene Signature Arrays enable analysis of hundreds of TaqMan® Gene Expression Assays on a 384-well micro fluidic card with minimal effort. TaqMan Gene Signature Arrays are readily available and delivered 2–4 days after ordering. They are packaged conveniently with two or four arrays per pack, making them cost effective, and just the right size for any experiment. Choose from pathway-specific pre-defined arrays such…
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results. Get gold standard TaqMan Assay performance for miRNA quantitation and profiling…
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance using Applied Biosystems TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
TaqMan® PreAmp Pools are pre-pooled sets of Applied Biosystems TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are availab…
The 2X Multiplex Master Mix, optimized for the Multiplex PCR using Hot-start Taq., is a Pre-Mix product effective in amplifying a number of DNAs with just a one-time PCR. Key features include: 1. Optimization for Multiplex PCR 2. Contains non-specific outputs 3. Enhanced user convenience 4. Applicable in various tests
All water products are certified to be nuclease free. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC; available in 100 ml, 500 ml and 1 liter sizes. Ambion also offers nuclease-free water that has not been DEPC treated for applications that may be negatively affected by residual DEPC (e.g., oocyte injection). Our nuclease-free, non-DEPC treated water is available in 500 ml…
GenomeWalker™ Kits provide a simple, PCR-based method for walking upstream or downstream in genomic DNA from a known sequence, such as an expressed sequence tag (EST). GenomeWalker allows you to take individual steps of up to 6 kb in genomic DNA using long-distance PCR. Walks can be extended simply by taking multiple steps using new primers based on the sequence obtained in previous steps.
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Transcription Factors and related genes reformatted onto an OpenArray plate. Kit includes: Open…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Inflammation and related genes reformatted onto an OpenArray plate. Kit includes: OpenArr…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Transcription Factors and related genes reformatted onto an OpenArray plate. Kit includes…
LightCycler® 1536 Real-Time PCR System from Roche Applied Science - High Throughput - Redefined Forge ahead into a new era in high-throughput real-time PCR with the new LightCycler® 1536 RT-PCR Instrument. Precisely engineered for miniaturization and parallelization, this latest innovation reinforces Roche Applied Science as a global expert in real-time PCR. Take advantage of over ten years of publication-proven expertise with…
Finnzymes’ novel approach, Direct PCR, saves you time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Finnzymes' Phusion® High-Fidelity DNA Polymerases and Phire® Hot Start DNA Polymerase. These unique fusion enzymes have been engineered to incorporate a double-stranded DNA binding domain that gives them extraordinary properties. Phusion and Phire DNA Polymerases are except…
For amplification of DNA directly from plant material. Finnzymes’ Phire® Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA isolation is required prior to the PCR. The kit is based on Phire® Hot Start DNA Polymerase, a specially engineered enzyme with a unique DNA binding domain. Phire DNA Polymerase is extremely robust and tolerant of many PCR inhibitors present in plant material. The kit incl…
Finnzymes’ Piko® Plate Illuminator is a state-of-the-art plate rack that provides a simple way to track the loading of reactants by illuminating the target well(s) from below with white LED lights. With 10 different pre-programmed loading patterns, this device can fulfill just about any protocol requirements. It accepts both 24-well and 96-well Piko® PCR Plates and can be used with all standard single channel, 8-channel or 1…
The Spartan DX-12™ is designed for non-batched PCR applications. It is a lightweight, stand-alone instrument. • 12-well sample capacity and dual-channel detection • Performs 2 or 3-temperature PCR programs • Small footprint maximizes bench space • Built-in Reverse Transcription (RT) program for RNA analysis • Compatible with leading reagents and kits
96-Well PCR® Rack - Removable hinged lid lies flat The Heathrow Scientific 96-Well PCR® Rack is perfect for pre- or post-PCR sampling. The sturdy polypropylene 96-Well PCR® Rack accommodates individual 0.2 mL tubes or strips of 8 or 12 tubes. Tube wells are easily identified with embossed numbers and letters. The 96-Well PCR® Rack is available in natural, single colors, or assorted colors (one each of blue, green, pink, yellow…
Reversible PCR® Rack - Removable hinged lid fits both sides Flip this polypropylene Reversible PCR® Rack to the size of tubes you need. The PCR side of the rack has 168 wells that hold 0.2 mL tubes. The wells accommodate 8- or 12-tube strips or individual tubes. The opposite side of the Reversible PCR® Rack has 40 wells that hold 0.5 mL tubes. Both sides of the rack have 12 wells for 1.5 mL tubes. Autoclavable. Available from…
Finnzymes’ Piko® Thermal Cyclers represent a fundamental improvement in PCR technology. Piko Thermal Cyclers incorporate novel technical solutions that allow significant reductions in PCR run times and the overall size of the instrument. Although merely half the size of other thermal cyclers, Piko Thermal Cyclers meet the highest criteria in performance expected from a thermal cycler and yet are able to complete a PCR protocol…
Bioneer’s AccuPower® PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer. AccuPower PreMix PCR master mix includes our super-processive “three times faster than Taq” Top DNA Polymerase for the fastest nucleic acid amplification. While Top DNA Polymerase is ideal for applications where you would normally use Taq, if your needs include…
AccuPower® HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart PCR master mix.Bioneer uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Bioneer’s Top DNA Polymerase is inhibited at lower temperatures (
AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized PCR Master Mix of CycleScript Reverse Transcriptase, a primer, and all of the other components for cDNA synthesis conveniently packaged in individual tubes. Simply add template RNA, and water. Then, the reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transc…
