Isolation
Bioneer’s AccuPrep® Plasmid MiniPrep DNA Extraction Kit allows for the extraction of highly-pure plasmid DNA from as E. coli bacterial cultures. A260/A280 ratio greater than 1.8 and Yields of up to 20 μg of high-copy plasmid DNA can be routinely attained using the AccuPrep Plasmid MiniPrep DNA Extraction Kit. The AccuPrep Plasmid MiniPrep DNA Extraction Kit was developed for plasmid DNA purification from cultured bacterial cel…
Bioneer’s AccuPrep® PCR Purification Kit allows rapid purification of DNA fragments (dsDNA and ssDNA) from PCR products and other enzymatic reactions. Purification of up to 10 µg of DNA fragments from PCR and other enzymatic products within 5 minutes with > 90% recovery are routine with the AccuPrep PCR Purification Kit. Size range is 100 bp - 10 kb, so common 20 - 40 mer oligonucleotides are easily removed. Elution volume can…
Bioneer’s AccuPrep® Gel Purification Kit allows rapid DNA gel purification of up to 10 μg DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gels as well as PCR products and enzymatic reaction mixtures. Fragments 70 bp – 10 kb can be isolated in less than 15 minutes with the AccuPrep Gel Purification Kit Recovery efficiencies are QC’ed to be greater than 70%. Elution volume can be as little as 30 μl when concen…
Bioneer’s AccuPrep® GMO DNA Extraction Kit allows rapid DNA extraction from agricultural and food products to detect genetically modified organisms (GMOs). The AccuPrep GMO DNA Extraction Kit yields large amounts of high-purity DNA; for example, 20~40 μg of plant DNA for 1 g of powdered bean, with an average A260/A280 ratio greater than 1.8. AccuPrep GMO DNA Extraction Kit works on the principle that DNA, In the presence of ch…
Bioneer’s AccuPrep® Stool DNA Extraction Kit allows for the rapid extraction of genomic, bacterial, viral, and parasite DNA from fresh or frozen stool. The AccuPrep Stool DNA Extraction Kit yields large amounts of contaminant free, high quality DNA routinely with average A260/A280 ratio greater than 1.8.The AccuPrep Stool DNA Extraction Kit quickly and conveniently extracts up to 5μg of DNA from 100~200 mg of stool. In the pre…
Bioneer’s AccuPrep® Viral RNA Extraction Kit is the ideal solution for rapid extraction of viral RNA from cell-free samples such as serum, plasma, CSF, urine, and from a variety of RNA virus, such as HIV, HAV, HCV and enteroviruses. The viral RNA extracted with the AccuPrep Viral RNA Extraction Kit can be directly used as a template for RT or RT-PCR. AccuPrep Viral RNA Extraction Kit obtained has an average A260/A280 ratio gre…
Bioneer’s AccuPrep® 96 Plasmid DNA Extraction Kit is used for the high throughput extraction of plasmid DNA from 96 different bacterial culture samples. Bacterial cultures can be grown and lysed in the AccuPrep 96-well blocks provided with the Plasmid Extraction kit. Purification is accomplished with the BioVac 96 Vacuum Manifold. Average A260/A280 ratio greater than 1.8. Maximum yield of 17 μg.The DNA extraction protocol used…
Bioneer’s AccuPrep® 96 Genomic DNA Extraction Kit allows for the simultaneous genomic dna purification from 96 samples of cultured cells, whole blood, buffy coat, lymphocytes, plasma, serum, and other biological fluids. Yield is sample dependent, but is typically 6 μg per 200 µl healthy whole human blood. The AccuPrep 96 Genomic DNA Extraction Kit is based on the principle of DNA binding to a silica membrane in the presence of…
Bioneer’s AccuPrep® 96 PCR Purification Kit is a high throughput PCR purification kit that allows PCR product purification from up to 96 PCR reactions at once. The AccuPrep 96 PCR Purification Kit allows purification of up 10 μg of DNA fragments from PCR and other enzymatic products within 25 minutes. The AccuPrep 96 PCR Purification Kit features recovery efficiencies up to 95%. Size range is 100 bp - 10 kb, thus 20-40 mer oli…
The Tissue RNA PreMate™ kit is designed for total RNA extraction from tissue, cultured cells, leukocytes or serum. The PreMate protocol is based on a Modified guanidinium salt-lysis method to optimally extract total RNA. Extracted total RNA is treated with PreMate’s RNase-free DNase to eliminate any genomic DNA. The RNA Isolated with the Tissue RNA PreMate kit is suitable for Northern analysis, dot blot hybridization, poly A s…
Viral RNA PreMate contains all of the required reagents for easy isolation of total RNA from RNA viruses such as HCV using the guanidinium salt-lysis method. Possible sources include tissue, cultured cells, leukocytes, and serum. The extracted RNA can be used for molecular cloning, RT-PCR, Northern analysis, dot blot hybridization, poly-A (+) selection, RNase protection assays, and other application that require pure RNA. Vira…
DNA PrepMate™ - M is used to extract genomic DNA for PCR from microorganisms with tough cell walls (such as Mycobacterium tuberculosis complex and Mycoplasma pneumoniae) using a standard microwave oven. DNA preparation is accomplished quickly and efficiently. Source materials can be sputum, CSF, urine, pleural fluid or tissue.
DNA PrepMate™ II uses an optimal binding buffer and silica matrix to effectively isolate DNA from bacterial cells, PCR products, gel fragments and enzymatic reaction mixtures. Examples include the purification of DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gel, high salt solutions, and lysed bacterial cells (using alkaline lysis). DNA preparation is accomplished quickly and efficiently. The size range fo…
The Montage Plasmid Miniprep96 Kit is a fast, easy-to-use kit for high-purity plasmid minipreps. Using a unique separation technology (patent pending), the Montage Kit follows a simple protocol that eliminates lengthy bind/elute methods and centrifugation steps to yield clean and reproducible DNA in 50% less time than traditional methods.The kit includes all the reagents and disposable materials you need to purify plasmid DNA…
