RNAi Reagents Products & Reviews

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E.coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.  Unique SET tags increase protein solubility  All three SET tags in every vector set  Gentle SBP purification tag generates high-yield, pure pro…

The ZAP Express and the Lambda ZAP-CMV vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. The ZAP Express vectors enable both prokaryotic and eukaryotic expression of your gene of interest. In vivo excision of the ZAP Express vector yields the PBK-CMV vector. The lacZ promoter drives prokaryotic expression, while the CMV immediate early promoter drives gene expression in mamm…

The ZAP Express and the Lambda ZAP-CMV vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. The ZAP Express vectors enable both prokaryotic and eukaryotic expression of your gene of interest. In vivo excision of the ZAP Express vector yields the PBK-CMV vector. The lacZ promoter drives prokaryotic expression, while the CMV immediate early promoter drives gene expression in mamm…

The ZAP Express and the Lambda ZAP-CMV vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. The ZAP Express vectors enable both prokaryotic and eukaryotic expression of your gene of interest. In vivo excision of the ZAP Express vector yields the PBK-CMV vector. The lacZ promoter drives prokaryotic expression, while the CMV immediate early promoter drives gene expression in mamm…

The ZAP Express and the Lambda ZAP-CMV vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. The ZAP Express vectors enable both prokaryotic and eukaryotic expression of your gene of interest. In vivo excision of the ZAP Express vector yields the PBK-CMV vector. The lacZ promoter drives prokaryotic expression, while the CMV immediate early promoter drives gene expression in mamm…

The ZAP Express and the Lambda ZAP-CMV vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. The ZAP Express vectors enable both prokaryotic and eukaryotic expression of your gene of interest. In vivo excision of the ZAP Express vector yields the PBK-CMV vector. The lacZ promoter drives prokaryotic expression, while the CMV immediate early promoter drives gene expression in mamm…

Ideal for small scale in vitro applications, screening DsiRNA duplex pricing includes affinity purification.

Trifectin is a proprietary cationic lipid formulation that has been optimized for delivery of IDT’s Dicer-Substrate siRNAs into a wide variety of cell types with minimal toxicity.

Lullaby® - siRNA transfection reagent is the ideal reagent dedicated to the transfection of siRNA. Based on an innovative technology, this new lipid-based reagent achieves extremely efficient siRNA delivery into cells. Lullaby® - siRNA transfection reagent protects siRNA from extracellular degradation and has an outstanding capacity to destabilize cells membrane, allowing delivery of important siRNA amounts into the cytosol. T…

siRNA designed and modified for greater specificity

Gene silencing in difficult-to-transfect cells guaranteed

The largest and most validated shRNA collection from the RNAi consortium (TRC). Merck's unique shRNA formats leverage the discovery potential of the trusted and proven TRC shRNA collection. When you partner with them, you gain access to their world class lentiviral production expertise and the formats and specifications for RNAi knockdown experiments at any scale.

Trusted TRC (The RNAi Consortium) content with shRNA targting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma

Easily identify hits from pooled shrna screens

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.

Developed at the Broad Institute, the MISSION ® TRC3 human whole genome pooled ORF library consists of over 17,000 unique ORF clones, encoding for over 14,000 genes used for overexpression screening

The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP™, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format

Compact Research Stainer

siPORT NeoFX’s lipid-based formulation enables the transfection of siRNA into adherent cells as they are subcultured — without increased cytotoxicity. From start to finish, successful gene silencing experiments can be completed in as little as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.

Ambion Anti-miR™ miRNA Inhibitors are chemically-modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miRNA molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.

Silencer Pre-Designed siRNAs are Ambion-designed siRNAs available for all human, mouse, and rat gene targets in the RefSeq database. These siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is provided with full sequence information. Silencer Validated siRNAs are Ambion-designed siRNAs that have been…

Ambion Silencer Select Pre-Designed and Validated siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Ambion Silencer® In Vivo Ready siRNAs undergo the additional purification and testing necessary for introduction of siRNA into animals. After HPLC purification and annealing, each siRNA is dialyzed to remove salt, sterile filtered, and endotoxin tested. All Silencer Pre-Designed and Validated siRNAs are available in this format, as are Custom Synthesized siRNAs.

Abmion Validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs are available for human, mouse and rat with the same chemical modifications for enhanced efficacy as in other Ambion Silencer Select siRNAs.