Protein Purification Products & Reviews

Sample preparation for ion chromatography

Filter particle-laden or viscous samples

This 13 mm syringe filter with a Hydrophilic Polyethersulfone membrane, for non-sterile filtration, has a 0.45 micron pore size used in preparation of aqueous & mild organic solutions prior to chromatography or other instrument analyses.

Millex-GP Filter for Samplicity G2, 0.22 µm, PES 33 mm, non-sterile

The PickPen 8-M magnetic tool offers the same benefits as the PickPen 1-M but allows parallel processing of 8 samples. The device is suitable for use with QuickPick™ kits in microplate applications.

The magnetic bead separation device Magnetic PlateX can be used with a Xiril pipetting workstation for automated nucleic acid purification based on the magnetic beads technology. Speed up the process of nucleic acid purification with a magnetic beads kit using the Magnetic PlateX on a Xiril robotic workstation. All pipetting steps are taken over by the robot and the plate gripper “Plate GripX” automatically carries out the tra…

Superior Bead Recovery and Low Levels of False Positives Ensure Assay Reproducibility. Smooth well wall provides efficient bead recovery, ensuring reproducible results lot after lot High performance membrane does not trap microspheres in the membrane matrix In serological assays, Supor® membrane effectively removes IgG complexes, thus reducing nonspecific reactivity of the microspheres and reducing false positives New well des…

Robust Chemical Resistance Provides Compatibility with Harsh Organics. Chemically resistant materials provide a stable platform to process samples in organic solvents Inert materials of construction ensure complete recovery of samples and low non-specific binding New well design results in faster, more uniform filtration rates across the plate and reduces hold-up volume Innovative outlet tip geometry provides direct flow of sa…

QuickPick™ GST kit for proteins, 48 preps (62411)The ready-to-use QuickPick GST glutathione affinity kit is intended for the purification of GST (glutathione-S-transferase)-tagged proteins. It is optimized for use with the PickPen 1-M magnetic tool, and at least 40 ug tagged protein can be purified per prep.

BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extracti…

AVOID FREEZE/THAW CYCLES. The ProteoExtract Subcellular Proteome Extraction Kit is a tool for the differential extraction of proteins from adherent or suspension tissue culture cells according to their subcellular localization. The sequential extraction steps yield fractions containing cytosolic proteins, plasma membrane and organelle proteins, soluble nuclear proteins, and finally cytoskeletal and nuclear matrix proteins. To…

PhosphoSafe™ Extraction Buffer extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. The reagent, an enhancement of the CytoBuster™ Protein Extraction reagent, includes four phosphatase inhibitors. PhosphoSafe is compatible with Western blotting, immunoprecipitations, and kinase assays.  

Purification of His•Tag fusion proteins by metal chelation chromatographyThe His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins.…

miVac is a range of centrifugal vacuum concentrators and freeze driers capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials. miVac concentrators come in 2 sizes, the smaller Duo and a larger Quattro. There is a choice of vacuum pump and rotor according to application and sample format.  To speed concentration built-in special runs for working with water and…

RFP -Trap® - use your fluorescence fusion as tag for biochemical studies Curiously RFP, a widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited RFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses. Superior results in a fraction of the time The ChromoTek RFP-Trap® overcomes these pro…

Fusion of proteins to Glutathione S-Transferase (GST) of Schistosoma japonicum is a common technique to increase solubility and expression level of a protein. Furthermore, GST-tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect GST fusion proteins. GST-Trap The GST-Trap is a highly specific and robust GST-binding protein coupled to ma…

DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…

Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles DNA and acts as a loading platform for multiple, transiently interacting factors participating in various DNA transactions. PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex n…

cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Roche's new proprietary nickel-chelate chemistry is compatible with commonly used reducing agents (such as DTT), chelating metalloprotease inhibitors (such as EDTA), and a wide range of buffer substances and salt conditions. The w…

Introducing an all-in-one centrifugal device for the protein purification workflow.Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workfl…

The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results. Features & Benefits • Complete set of materials for up to…

cOmplete His-Tag Purification Column - Prepacked columns with EDTA-compatible resin for the purification of poly-histidine-tagged proteins. cOmplete His-Tag Purification Columns are available in 2 different sizes and prepacked with cOmplete His-Tag Purification Resin. cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for convenient single-step purificati…

The PureProteome Protein G Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability. PureProteome Protein G magnetic beads are a powerful system to isolate antibodies faster, easier, and with more reproducibility then ever before. PureProteome Protein G magnetic beads purify your sample with the highest binding capacity of any magnetic beads available.…

Purification Consumables and Custom Products.We understand that your purification challenges are unique. That’s why we offer you a variety of options to help you solve them. Whether you seek the up-front cost savings and versatility of packing your own columns, the consistent quality and time savings of pre-packed columns, or the ease associated with having us purify material for you; Sigma-Aldrich is your purification partner…