Gene Expression and Molecular Cloning Products & Reviews

Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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siPORT™ siRNA Electroporation Buffer/Kit

Thermo Fisher Scientific

Electroporation with an effective buffer and optimized conditions can provide a highly efficient method for transfecting primary and hard-to-transfect cells with siRNAs. The siPORT™ siRNA Electroporation Buffer/Kit can be used with multiple types of electroporators to ensure highly efficient delivery of siRNA into cells while maintaining high cell viability.

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siPORT™ XP-1 Transfection Agent

Thermo Fisher Scientific

siPORT XP-1 is an easy-to-use reagent that efficiently delivers both plasmid DNA and PCR products into a variety of mammalian cell types. Comprised of a proprietary formulation of polyamines, the reagent exhibits low toxicity and can be used both in the presence or absence of serum. siPORT XP-1 is particularly straightforward to use. Reagent/DNA complexes can be added directly to growing cells in serum-containing culture med…

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DyNAmo™ cDNA Synthesis Kit

Finnzymes Oy

The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications. This kit can be used in conjunction with Finnzymes' DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed…

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Phusion™ Site-Directed Mutagenesis Kit

Finnzymes Oy

The Phusion™ Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with Quick T4 DNA Ligase. The resulting…

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DNAfectin

NBS Biologicals Ltd.

The Best Transfection Reagent. Applicable to cells that are difficult to transfect. DNAfectin™ is specially formulated with multiple cationic polymers. Application: Transfection of DNA into cultured eukaryotic cells.

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Thermo Scientific Verso cDNA Synthesis Kits

Thermo Fisher Scientific

The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…

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