Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
TaqMan® Gene Sets are pre-defined lists of genes grouped by pathway or disease target class which can be ordered separately, as a custom plate, or configured directly onto TaqMan® Arrays to enable you to perform hundreds of real-time PCR reactions simultaneously on the Applied Biosystems 7900HT Fast Real-Time PCR System. Simple to implement, TaqMan Gene Sets are customizable, and when placed on a TaqMan Array, require mini…
TaqMan® Gene Signature Arrays enable analysis of hundreds of TaqMan® Gene Expression Assays on a 384-well micro fluidic card with minimal effort. TaqMan Gene Signature Arrays are readily available and delivered 2–4 days after ordering. They are packaged conveniently with two or four arrays per pack, making them cost effective, and just the right size for any experiment. Choose from pathway-specific pre-defined arrays such…
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results. Get gold standard TaqMan Assay performance for miRNA quantitation and profiling…
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance using Applied Biosystems TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
TaqMan® PreAmp Pools are pre-pooled sets of Applied Biosystems TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are availab…
The TaqMan Sample-to-SNP Kit provides a streamlined protocol for performing TaqMan® chemistry-based genotyping analysis from any sample with a single kit. The kit is comprised of two parts: the DNA Extract All Lysis Reagents and the TaqMan® GTXpress™ Master Mix. The DNA Extract All Lysis Reagents reduce prolonged procedures for the release of real-time PCR-ready DNA to a 5 minute protocol. They can process a wide variety of…
Optimized to achieve over 50% transfection efficiency without sacrificing cellular health, each kit includes an optimized protocol, Prostate Reagent and Prostate Boost Reagent in sufficient quantities to conduct up to 500 transfections in 35mm wells.Features include:• Reduces levels of cell damage when compared to liposmal-based transfection reagents • Transfection can be conducted in serum-containing media to save time and el…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Inflammation and related genes reformatted onto an OpenArray plate. Kit includes: OpenArr…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Transcription Factors and related genes reformatted onto an OpenArray plate. Kit includes…
febit offers a broad variety of Geniom® Biochips useable with a Geniom RT Analyzer or febit’s Analytical Services. Customers can choose from the growing portfolio of Geniom Biochips or order Custom Geniom Biochips manufactured exactly to precise specifications. Geniom Biochips are available with validated and optimized probe design for specific applications such as: • microRNA Profiling • mRNA Profiling • HybSelect, sequence c…
