Gene Expression and Molecular Cloning Products & Reviews

Direct translocation of proteins into living cells

Pre made lentiviral particles for flourescent proteins

FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.

TOPglow (TCF reporter plasmid): Transfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box and Luciferase open reading frame.

The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is introduced to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell line…

A highly efficient method of gene trasfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 fold in some cells, by including polybrene during the infection. Polybrene with DMSO shock is also used to mediate DNA transfer into a variety of cell types, such as: CHO, chicken embryo fibroblases, NIH3T3 cells, and myeloid cells.

The magnefect-nano from nanoTherics is full-feature, fully flexibly, easy-to-handle, fast and efficient cell transfection device. The system uses a patented technology which applies proprietary, oscillating magnet arrays to greatly improve transfection speed and efficiency. This results in significantly faster and higher transfection efficiencies over the traditionally used lipid-based and static magnet assisted transfection a…

Create your own custom microarray using eArray, Agilent's online design application, and focus on the targets of interest for your specific research needs.

The Agilent SurePrint G3 Human Exon Microarrays include probes targeting individual exons, making it possible to look beyond the gene to more subtle but relevant expression changes at the exon level. Agilent Exon Microarrays are the fast, simple, and cost-effective choice for exon expression analysis, with capabilities to drill down into important RNA changes at the exon level.

Agilent’s Low Input Quick Amp WT Labeling Kit reliably amplifies and labels whole transcripts for eukaryote and prokaryote samples, with a low sample input range of 25-100 ng total RNA.

Agilent Gene Expression Microarrays provide a comprehensive coverage of genes and transcripts to study gene expression profiling for a wide variety of organisms and species.

The Agilent SurePrint G3 Mouse Exon Microarrays include probes targeting individual exons, making it possible to look beyond the gene to more subtle but relevant expression changes at the exon level. Agilent Exon Microarrays are the fast, simple, and cost-effective choice for exon expression analysis, with capabilities to drill down into important RNA changes at the exon level.

SurePrint G3 Mouse Gene Expression Microarrays provide comprehensive coverage of genes and transcripts using the latest annotation databases. The new SurePrint G3 Mouse Gene Expression v2 Microarray features complete coverage of establish RefSeq coding transcripts (NM) from the latest build and updated long non-coding RNA (lncRNA) content to ensure relevant research.

Agilent’s Quick Amp Labeling Kits reliably amplify and label target RNA for the robust generation of cRNA.

The Agilent SurePrint G3 Rat Exon Microarrays include probes targeting individual exons, making it possible to look beyond the gene to more subtle but relevant expression changes at the exon level. Agilent Exon Microarrays are the fast, simple, and cost-effective choice for exon expression analysis, with capabilities to drill down into important RNA changes at the exon level.

SurePrint G3 Rat Gene Expression Microarrays provide comprehensive coverage of genes and transcripts using the latest annotation databases. The new SurePrint G3 Rat Gene Expression v2 Microarray features complete coverage of RefSeq transcripts from the latest build to ensure relevant research.

The Agilent Protein 80 and Protein 230 kits provide a reliable way to assess protein concentration, identity, and purity. It eliminates handling of SDS-PAGE slab gels, staining or imaging steps.

The Agilent DNA Fish ID solution uses advanced software to achieve more accurate results – quickly and easily. Key to the process is the Agilent 2100 Bioanalyzer and RFLP Decoder Software performs RFLP pattern matching, which eliminates the need for manual calling

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin.

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E. coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.  

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.