Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format. What benefits are important for your work? Efficient transfection in single cuvette format? Up to 90% efficiency with plasmid DNA…
The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.
Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunoprecipitation (ChIP) pull-down coupled with powerful DNA microarray location analysis. Beyond embryonic and adult development gene regulation, Agilent™s ChIP-on-chip model systems effectively elucidates the vital biological processes. Probe number depends on species 4x44k and…
The Agilent Rabbit Microarray delivers accurate, consistent and efficient gene expression profiling of rabbit samples. The transcriptome-wide microarrays enable scientists to study gene expression profiling of Oryctolagus cuniculus samples at the global level and enhance the understanding of gene function.
In combination with the included Enhancer Solution, the reagent efficiently delivers functionally active proteins, antibodies and peptides into the cytoplasm of cells. Proteins of different sizes and net charge (different pI) can be delivered into various cell types, including primary, adherent and suspension cells (see Fig. 1, 2 and list of cells below). The reagent can be used in the presence or absence of serum. TurboFect P…
The Yeastmaker Yeast Transformation System 2 provides a high-efficiency polyethylene glycol (PEG)/LiAc-based method for preparing and transforming competent yeast cells. The Yeast Transformation System 2 protocol provides a higher and more reliable transformation efficiency than many other commonly used methods as a result of specially formulated "YPD Plus" recovery medium, which results in more surviving transformed colonies…
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.
The gold standard in bead-based NGS cleanup and size selection
The DNA Ligation Kit Mighty Mix is a single solution, ready-to-use 2X ligation mix that enables high efficiency ligations, particularly for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The basic ligation reaction can be quickly performed, either for 5 minutes at 25°C or for 30 minutes at 16°C, with equal results for both circular and linear DNA ligations. The 2X Ligation Mix is c…
