RNA Interference Products & Reviews

Tailored transfection reagents for a wide variety of RNA and cells. High-efficiency delivery, low cellular toxicity, broad dynamic range

Crucial to any gene silencing or microRNA modulation experiment.  RNAi controls allow accurate interpretation for reliable, reproducible results.

Successful gene silencing in your cells of interest

Tightly controlled gene silencing with the most advanced and flexible single-vector inducible shRNA.

DsiRNAs are 27mer duplex RNAs that demonstrate increased potency in RNA interference compared to traditional, 21mer siRNAs. Proprietary design rules produce optimized DsiRNAs that are available only from IDT.

Antisense oligonucleotides (ASOs) are short oligonucleotides that localize to the nucleus and provide a pathway for gene silencing by the RNase H pathway. Phosphorothioate (PS) linkages are available to confer nuclease resistance and, therefore, enhance intracellular stability.

These steric blocking oligonucleotides hybridize to mature miRNAs and inhibit their function.

The largest and most validated shRNA collection from the RNAi consortium (TRC). Merck's unique shRNA formats leverage the discovery potential of the trusted and proven TRC shRNA collection. When you partner with them, you gain access to their world class lentiviral production expertise and the formats and specifications for RNAi knockdown experiments at any scale.

Trusted TRC (The RNAi Consortium) content with shRNA targting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma

Easily identify hits from pooled shrna screens

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.

Developed at the Broad Institute, the MISSION ® TRC3 human whole genome pooled ORF library consists of over 17,000 unique ORF clones, encoding for over 14,000 genes used for overexpression screening

The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP™, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format

Compact Research Stainer

Biotin labelling of protein, amine reactive.  Only 1 hour to get conjugates, all processes in a single filtration tube, high recovery of conjugates, applicable for 50-200 ug IgG

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression.

Obtain a reliable measure of GAPDH enzyme activity in cultured human, mouse, or rat cells in less than 30 minutes using a microplate fluorometer.

siPORT NeoFX’s lipid-based formulation enables the transfection of siRNA into adherent cells as they are subcultured — without increased cytotoxicity. From start to finish, successful gene silencing experiments can be completed in as little as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.

Ideal for small scale in vitro applications, screening DsiRNA duplex pricing includes affinity purification.

Trifectin is a proprietary cationic lipid formulation that has been optimized for delivery of IDT’s Dicer-Substrate siRNAs into a wide variety of cell types with minimal toxicity.

Ambion Anti-miR™ miRNA Inhibitors are chemically-modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miRNA molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.

Silencer Pre-Designed siRNAs are Ambion-designed siRNAs available for all human, mouse, and rat gene targets in the RefSeq database. These siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is provided with full sequence information. Silencer Validated siRNAs are Ambion-designed siRNAs that have been…

Ambion Silencer Select Pre-Designed and Validated siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Ambion Silencer® In Vivo Ready siRNAs undergo the additional purification and testing necessary for introduction of siRNA into animals. After HPLC purification and annealing, each siRNA is dialyzed to remove salt, sterile filtered, and endotoxin tested. All Silencer Pre-Designed and Validated siRNAs are available in this format, as are Custom Synthesized siRNAs.