Protein Expression Products & Reviews

Three different strains that provide varying levels of expression control with T7 promoter-driven vectors, such as the pCAL and pET vectors.BL21 derivatives for tight expression control Express toxic proteins Use BL21, BL21-Gold or BL21-CodonPlus cells High-level protein expression Use for established expression constructs Economical alternative

The easiest of HPLC systems to operate, without compromising build quality.

BL21(DE3) is a chemically competent  E. coli  cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Detect low-abundant proteins at the femtogram/mL level with the sensitivity and speed of the SMCxPRO™ immunoassay system – a powerful, compact and affordably priced protein quantifier. Discovery depends on detection. When the success of your research requires finding and quantifying low-level proteins, you need equipment you can rely on to detect your target.  This compact, benchtop instrument will fit seamlessly into your…

Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.

Maximum protein recovery

The TNT® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic cell-free protein expression: a single-tube, coupled transcription/translation system. The TNT® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results.

High Yield Cell-Free Protein Expression System  

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin.

BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E. coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.  

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

Method completed in less than one day versus 2 days with restriction enzymes

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.

The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.

Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.

The Mammalian Two-Hybrid Assay Kit is a powerful method for detecting protein-protein interactions in vivo in mammalian cells. The two-hybrid assay kit utilizes hybrid genes to detect protein-protein interactions via the activation of reporter-gene expression.  Detects protein-protein interactions in mammalian cells  Works well in a variety of mammalian cell lines  Confirms suspected interactions between two proteins or i…

The pDual and pDual GC expression vectors are designed for high-level expression of heterologous genes in both eukaryotic and prokaryotic systems.High-level protein expression in bacterial or mammalian systems Saves time by eliminating the need to subclone Express protein as a native or tagged fusion protein Epitope tagged for simple detection and affinity purification Clone seamlessly without compatible restriction sites Avai…

Alpha Laboratories offers a range of products to aid proteomic investigations from separation to sequencing along with the Phos-tag™ series specifically designed for analysis of protein phosphorylation. Products include: Automated protein sequencing chemicals and reagents Enzymes and inhibitors Mass spectrometry protein analysis Phos-tag™ Protein electrophoresis reagents Glycobiology Exosome Isolation Kit…