Streptavidin Magnetic Beads
Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.
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Simple SOP, long lasting
Microfluidics
I like them. The SOP is simple. The only thing that needs to be improved is their affinity for DNA.
Review Date: 26 Mar 2019 | New England Biolabs Inc.
Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.
- The strength of the biotin-streptavidin interaction, an association constant (Ka) of 1015 M-1, coupled with the low non-specific binding of streptavidin permits captured substrates to be useful as ligands in subsequent experiments including mRNA isolation, nucleic acid enrichments and the capture of primary or secondary antibodies
- Other applications include 5-hmC detection and analysis, methylated DNA analysis, and solution-phase panning in phage display experiments
- Binding capacity: ≥30 µg biotinylated antibody per mg and >500 pmol of single-stranded 25 bp biotinylated oligionucleotide per mg
- High binding capacity and low non-specific binding of proteins make this bead ideally suited for protein isolation applications
- Quick, small-scale purification of biotinylated proteins affords higher throughput and efficiency
