3D High-Content Screening of Liver Spheroids

More accurate in vitro toxicity assays are required to detect adverse drug effects, such as drug induced liver injury (DILI), in earlier phases of drug development. 3D cell models demonstrate the formation of functional bile canaliculi and have increased albumin secretion and CYP expression, as well as longer stability in culture compared with two dimensional (2D) cultures, making them ideal for DILI studies. Here, efficacious drug screening is demonstrated by combining Corning^® Hepatocells with Corning spheroid microplates to create the 3D cell cultures necessary for testing.