3D Spheroid Culture and Drug Testing Using a Novel Hanging Drop Culture Plate

3D cell culture systems are essential to acquiring physiologically‐relevant results. However, scaling up and maintaining reproducibility of 3D cell cultures have been difficult due to lack of standardized tools and methods that are simple to use and compatible with existing equipment. This scientific poster present a novel 3D cell culture platform that enables uniformly-sized spheroids to be generated and tested in a 384-microplate format and using high throughput screening instruments, such as plate readers, liquid handling robots, and automated imaging and analysis systems.