A Functional BRET Assay for 7TM Receptors Using the Mithras LB 940 Multimode Plate Reader

7TM (seven transmembrane segment) receptors, also known as G-protein coupled receptors, constitute the largest super-family of proteins in our organism and are targets for a large number of intercellular messengers such as hormones and transmitters. 7TM receptors are under the focus of extensive investigation as they represent both potential and established drug targets. The use of BRET has previously been limited by lack of sensitive instrumentation. However, this application note demonstrates the use of the multimode plate reader Mithras LB 940, allowing for screening of virtually all 7TM receptors independent of their signaling pathway.