A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein Quantitation

Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots. Download this application note to learn how stain-free total protein measurement serves as a more reliable loading control than housekeeping proteins.