A Method for High Quality Digoxigenin-Labeled RNA Probes for In Situ Hybridization

Digoxigenin (DIG)-labeled RNA antisense probes are widely used for in situ hybridization due to their high sensitivity and specificity. DIG-labeled RNA probes are also stable for more than a year, making them ideal for long-term studies with high consistency and low technical variation. In this application note read how DIG-labeled probes were used to investigate the localization of human hepatocyte growth factor (hHGF), a cell surface cytokine binding the c-MET-receptor, implicated in the tyrosine kinase signaling cascade causing tumor genesis. Her2 was also examined which binds and stabilizes numerous EGF-receptor-ligand-interactions in downstream signaling pathways. The overexpression of both genes has been demonstrated in variety of cancers.