A Method to Streamline and Miniaturize Nextera™ Library Preparation for Illumina Next-Gen Sequencing

The ongoing evolution of next-generation sequencing (NGS) technologies has reduced sequencing costs by orders of magnitude and significantly increased throughput. Library preparation costs form a large proportion of the total cost of NGS, but there is potential to reduce these costs via miniaturization. This application note investigates two possible approaches to increase cost saving in library preparation workflows, enabled with Labcyte’s Echo 525 Acoustic Liquid Handler.