A New Quantitative Microplate-Based Multiplex Technology

In this application note from R&D Systems, a method is described to quantify several different proteins in an experimental sample. The Mosaic ELISA Human Cytokine Panel 1 is used to detect multiple analytes simultaneously in PHA-treated human peripheral blood mononuclear cells (PBMCs). By combining the design and specificity of the traditional ELISA with multiplex technology, the Mosaic ELISA Human Cytokine Panel 1 and Human Growth Factor Panel 1 are designed to measure the levels of up to 8 different cytokines or growth factors simultaneously in a single sample of cell culture supernate, serum, or plasma.