A Novel Instrumental Strategy for Parallel Reaction Monitoring of Intact Proteins with an Orbitrap Analyzer

This application note describes a novel approach for parallel, concurrent selection of multiple precursors and multiple charge states of intact protein precursor species followed by higher-energy collision induced dissociation (HCD) and high mass accuracy detection of fragment species, yielding highly confident identifications. A time-of-flight mass selector is used in place of mass selection quadrupole filter in a quadrupole-Orbitrap hybrid Mass Spectrometer.