A Novel Solid-State ChIP Platform in High Throughput Format – Chromatrap® 96 HT

This application note demonstrates the potential of Porvair Sciences’ Chromatrap® 96 HT in the field of epigenetics. Sensitive enrichment of gene loci following immunoprecipitation with antibodies directed against transcription factors, alongside indicative histone marks, is clearly shown here to be highly selective, demonstrating excellent reproducibility. Parallel observation of transcription factor recruitment and histone methylation in multiple cell lines is described, demonstrating the capability of the platform to generate vast quantities of high quality, comparable data in a single one-day experiment.