A Novel, Substrate-Agnostic Class III HDAC Activity Assay

Read this note featuring an application for epigenetics research. An assay platform that enables the analysis of sirtuin activity using virtually any appropriate substrate has been developed and is outlinesd here. This new SIRTainty™ class III HDAC assay is a flexible, reliable, homogeneous, no-wash assay for quantifying sirtuin activity. Based upon novel, patent-pending technology, this easy-to-perform assay is coupled to nicotinamidase, which catalyzes breakdown of nicotinamide generated upon cleavage of NAD+ during sirtuin-mediated deacetylation of a substrate.