A test panel for rapid Monkeypox virus detection using multiplex probe-based qPCR

In this application note, PCR Biosystems describes a reliable and rapid (40-70 min) multiplex assay for the detection of Monkeypox and the identification of the viral type. Monkeypox is an infectious disease in humans and other animals, which manifests with fever, swollen lymph nodes, and a rash that forms blisters and crusts over. It may take up to 21 days to show symptoms from initial exposure, and they may last for two to four weeks. The disease is caused by the Monkeypox virus (MPXV), a zoonotic DNA virus belonging to the genus Orthopoxvirus, the same genus of variola virus (known to cause smallpox). There are teo types of MPXV affecting humans, of which clade II (formerly known as the West African clade) causes a less severe disease than the Central African / Congo basin (clade I) type.