AcGFP and mCherry Calibration Beads for Flow Cytometry

Commercially-available calibration beads are an indispensable tool when preparing flow cytometers for experiments using fluorescent dyes (e.g. FITC); however, existing fluorescent dye beads are not suitable for instrument calibration if the cells being analyzed express fluorescent proteins. To solve this problem, Takara has developed calibration beads labeled with either the red fluorescent protein mCherry or the green fluorescent protein AcGFP, which has spectral properties almost identical to EGFP. This poster demonstrates that the fluorescent protein Flow Cytometer Calibration Beads are easy to use, and perform equally well on a variety of flow cytometer platforms.