Advanced Application of CD Measurement for Pharmaceutical Research

Human serum albumin (HSA) is the most abundant protein in blood plasma. Chirality resulting from an achiral substance interacting with a chiral substance is called induced CD. This application note presents the measurement of CD induced by the interaction of HSA titrated with 3,5-diiodosalicylic acid and subsequent analysis of the dissociation constant, using Jasco’s J-1500 CD Spectrometer.