Advanced nuclease applications in lentiviral vector bioprocessing

Explore how alternative nucleases, salt active nuclease (SAN) and medium-salt active nuclease (M-SAN), are evaluated in lentiviral vector (LV) manufacturing to improve gene therapy product quality. Compared to traditional nucleases, SAN and M-SAN achieved enhanced vector recovery, reduced aggregation, and significantly lower residual DNA in the final product. These findings support the use of alternative nucleases to address critical quality needs in LV manufacturing.