An Automated, High-Throughput Library Construction Protocol with Benefits for Low-Input Applications

This poster describes a versatile, automated method for high-throughput Illumina® library construction using the KAPA HTP Library Preparation Kit on the Biomek® FX Laboratory Automation Workstation (Beckman Coulter). Data presented confirms that the automated method and KAPA library construction reagents offer robust, high-throughput library construction over a range of DNA inputs and sample types, and yields sequence data comparable or better than that achieved with a highly optimized manual method, whilst employing fewer cycles of pre-capture amplification.