An Automated, High Throughput Method for the Quantification of Endogenous Cellular Kinase Activity

Cellular kinases play an important role in relaying signals from activated receptors residing at the cell membrane to the interior of the cell, through signal transduction. The cellular processes in which they are associated include angiogenesis, cell growth, cell migration, and apoptosis. In this application note from BioTek, a method that uses HTRF® technology to quantify cellular kinase activity of VEGFR2 and STAT3 without overexpression of kinase or its substrate is described. Automation of all assay component dispense steps were performed in a high throughput format using a non-contact dispenser.