Analysis of Migration Using siRNA and the Oris™ Cell Migration Assay

To study migration in cell lines siRNA and the ORIS™ TriCoated Cell Migration Assay were combined. This assay from Platypus offers certain advantages over traditional migration assays. U2OS cells were transfected with a control non-targeting siRNA or with three different siRNA pools targeting specific proteins. Fluorescence-based signal curves for tissue culture treated, Collagen I and Fibronectin coated wells were monitored with a POLARstar Omega microplate reader from BMG LABTECH.