Analysis of the Circadian Clock and Plant Growth with NightSHADE

In this application note by BERTHOLD TECHNOLOGIES, gene expression in Arabidopsis is analysed using promoter::firefly Luciferase (LUC) reporter constructs and the NightSHADE imaging chamber. Analysis of circadian clock associated 1 (CCA1) and chlorophyll a/b-binding protein (CAB) constructs showed that CCA1 expression preceded activation of CAB promoter, consistent with CCA1 protein playing a positive role in CAB gene expression.