Approaching Single-Cell Sequencing by Understanding NGS Library Complexity and Bias

This application note describes the sequence coverage performance and preservation of molecular complexity of next generation sequencing (NGS) libraries generated from human and microbial genomic DNA using the Accel-NGS™ 2S DNA Library Kit for whole-genome sequencing on the Illumina® platform. Comparisons to the leading commercially available methods are also presented. From the lowest input DNA quantity supported for PCR-free libraries, this new technology demonstrated more than 50% higher library complexity.