Assessing Epigenetic Enzyme Activity Using HTRF® Epigenetic Assays from Cisbio with the PHERAstar FS from BMG LABTECH

This application note describes the verification of the performance of a Cisbio HTRF® based assay using the PHERAstar FS from BMG LABTECH. This assay allows users to detect the activity of G9a, a histone methyltransferase that methylates histone H3 on lysine 9 (H3K9). G9a mediated H3K9 methylation has been shown to be involved in transcriptional repression of developmental genes which is essential for early embryogenesis. In addition G9a has been shown to have an oncogenic function based on its ability to sustain cancer cell proliferation. Therefore, G9a is an excellent example of the type of epigenetic target that is of high importance in drug development.