Assessing Parallelism Using Parallel-Line Analysis in MARS

In this application note, a parallel-line analysis (PLA) is applied to inhibitor binding data of a prostaglandin D synthase (hematopoietic-type) and a fluorescence polarization (FP)-based inhibitor screening assay estimates the relative binding potencies. PLA is a common approach to assess parallelism of dose-response curves which are often obtained in biological assays.