Assessment of Drug Effects on Cardiomyocyte Physiology Using Human iPSC-Derived Cardiac Spheroids

Cell models are becoming more complex in order to better mimic in vivo microenvironments and provide greater predictivity for compound efficacy and toxicity. There is an increasing interest in exploring the use of three-dimensional (3D) spheroids for modelling tissue biology and toxicity assessment. Development of quantitative assays in higher throughput using 3D cultures is an active area of investigation. In this study, we have developed methods for the formation of 3D spheroids derived from human induced pluripotent stem cells (iPSC).