Authentication of Vanilla Extracts by Convergence Chromatography

The Waters® ACQUITY UPC2 System utilizes CO2 mobile phases along with organic co-solvent and additives to provide orthogonal selectivity to that of RPLC. For the analysis of vanilla extracts, this separation technique provides greater retention of highly polar, secondary components of vanillin while providing adequate retention and identification of non-polar adulterants. In addition, this chromatographic technique allows for improved efficiency and lower solvent usage than traditional RPLC methods, while providing a high-throughput, sensitive screening method for the analysis of vanilla extracts.