Automated Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cell-Mediated Cytotoxicity Utility

This note demonstrates the automation of two HTRF^® assays for the characterization and selection of potent antibody drug candidates. The first assay quantifies the binding affinity of antibodies to their target antigen, on live cells. The second assay measures the affinity of the antibody Fc portion to the CD16 receptor also on live cells.