Automated counting of isolated nuclei: Transform your single-cell genomics workflow
24 Jul 2023The requirement for ever-greater focus and precision has driven the development of improved single-cell sequencing analysis technologies. Techniques such as RNA sequencing and ATAC sequencing require intact single nuclei, so the ability to isolate individual nuclei from sample tissue is therefore critical to the success of these sequencing approaches. Isolating nuclei that are non-clustered and free from debris is critical both to successful library preparation and the ability to count nuclei accurately.
To help you achieve isolation of nuclei for your single-cell genomics workflows, this expert guide outlines:
- Automated counting of isolated nuclei
- Best practices for cell counting
- Improving cell counting accuracy
It will also cover two case studies – frozen brain tissue and plant protoplasts – including technical notes.
Download your free copy to learn more about the key features and applications of the CellDrop™ FL Automated Cell Counter from DeNovix as well as how the DeNovix DirectPipette™ technology is designed to eliminate plastic slides and cumbersome hemocytometers from routine cell counting.