Automated Directional RNASeq Library Preparation Using the Biomek FXP Liquid Handler

The purpose of this tech-note is to describe the automation of the New England Biolabs NEBNext^® Ultra Directional RNA Library Prep Kit for Illumina on the Beckman Coulter Biomek FXP automated liquid handler. The Biomek NEB Ultra Directional RNA Method was designed to work with NEBNext Ultra Directional RNA Library Prep Kit for Illumina protocol, and provides a solution for simultaneously constructing up to 96 individually bar-coded directional RNASeq libraries per method run.